SARS Nucleocapsid protein N1 Recombinant Protein | SARS N recombinant protein
SARS Nucleocapsid protein N1 Recombinant
Purity
>95%, as determined by SDS-PAGE.
Synonyms
SARS Nucleocapsid protein N1; SARS Nucleocapsid protein N1 Recombinant; sars; nucleocapsid; SARS N recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.
Sequence Length
422
Application Notes
SARS antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.
Host Note
DNA sequence encoding immunodominant fragment of SARS Nucleocapsid protein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant SARS N encodes the N terminal immunodominant sequences of the SARS Nucleocapsid protein.
Recombinant SARS N contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Recombinant SARS N contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Product Categories/Family for SARS N recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,025 Da
NCBI Official Full Name
nucleocapsid protein
UniProt Protein Name
Nucleoprotein
UniProt Gene Name
N
UniProt Synonym Gene Names
NC; Protein N
UniProt Entry Name
NCAP_CVHSA
Uniprot Description
Function: Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid
By similarity.
Subunit structure: Monomer and oligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with the M protein
By similarity.
Subcellular location: Virion. Host cytoplasm › host perinuclear region. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus. Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding. Not found in the nucleolus. Ref.22
Sequence similarities: Belongs to the coronavirus group 2a nucleocapsid protein family.
Similar Products
Product Notes
The SARS N n (Catalog #AAA553125) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. SARS antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems. . Researchers should empirically determine the suitability of the SARS N n for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS Nucleocapsid protein N1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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