Genome polyprotein Recombinant Protein | N-pro recombinant protein
Recombinant Classical swine fever virus Genome polyprotein
NCBI and Uniprot Product Information
Uniprot Description
Function: E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane
By similarity.P7 forms a leader sequence to properly orient NS2 in the membrane
By similarity.Uncleaved NS2-3 is required for production of infectious virus
By similarity.NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus
By similarity.NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase
By similarity.NS4A is a cofactor for the NS3 protease activity
By similarity.RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome
By similarity.
Catalytic activity: Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites.Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).NTP + H2O = NDP + phosphate.ATP + H2O = ADP + phosphate.
Subunit structure: The E(rns) glycoprotein is found as a homodimer; disulfide-linked
By similarity. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers
By similarity.
Subcellular location: E(rns) glycoprotein: Host membrane; Peripheral membrane protein. Note: The C-terminus membrane anchor of Erns represents an amphipathic helix embedded in plane into the membrane
By similarity.Envelope glycoprotein E2: Host cell surface
By similarity. Cysteine protease NS2: Host membrane; Multi-pass membrane protein
Potential.
Post-translational modification: The E(rns) glycoprotein is heavily glycosylated
By similarity.The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C.Cleavage between E2 and p7 is partial
By similarity.
Sequence similarities: Belongs to the pestivirus polyprotein family.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 1 peptidase C53 domain.Contains 1 peptidase C74 domain.Contains 1 peptidase S31 domain.Contains 1 RdRp catalytic domain.
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Product Notes
The Genome polyprotein n-pro (Catalog #AAA1239532) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 3181-3898. The amino acid sequence is listed below: SNWVMQEENK QSNLTPLFEE LLQQCPPGGQ NKTAHMVSAY QLAQGNWMPT SCHVFMGTIS ARRTKTHPYE AYVKLRELVE EHKMKTLCPG SSLRNDNEWV IGKIKYQGNL RTKHMLNPGK VAEQLHREGH RHNVYNKTIG SVMTATGIRL EKLPVVRAQT DTTNFHQAIR DKIDKEENLQ TPGLHKKLME VFNALKRPEL ESSYDAVEWE ELERGINRKG AAGFFERKNI GEILDSEKIK VEEIIDNLKK GRNIKYYETA IPKNEKRDVN DDWTAGDFVD EKKPRVIQYP EAKTRLAITK VMYKWVKQKP VVIPGYEGKT PLFQIFDKVK KEWDQFQNPV AVSFDTKAWD TQVTTNDLEL IKDIQKYYFK KKWHKFIDTL TMHMSEVPVI TADGEVYIRK GQRGSGQPDT SAGNSMLNVL TMVYAFCEAT GVPYKSFDRV AKIHVCGDDG FLITERALGE KFASKGVQIL YEAGKPQKIT EGDKMKVAYQ FADIEFCSHT PIQVRWSDNT SSYMPGRNTT TILAKMATRL DSSGERGTIA YEKAVAFSFL LMYSWNPLIR RICLLVLSTE LQVKPGKSTT YYYEGDPISA YKEVIGHNLF DLKRTSFEKL AKLNLSMSVL GAWTRHTSKR LLQDCVNMGV KEGNWLVNAD RLVSSKTGNR YVPGEGHTLQ GRHYEELALA RKQINSFQGT DRYNLGPIVN MVLRRLRVMM MTLIGRGV. It is sometimes possible for the material contained within the vial of "Genome polyprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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