Myrmecia pilosula M-myrmeciitoxin-Mp2b Recombinant Protein
Recombinant Myrmecia pilosula M-myrmeciitoxin-Mp2b
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Myrmecia pilosula M-myrmeciitoxin-Mp2b; Recombinant Myrmecia pilosula M-myrmeciitoxin-Mp2b; DELTA-myrtoxin-Mp1a B chain; Mp1a B chainPilosin-3 subunit b; Pilosulin-3b; Myrmecia pilosula M-myrmeciitoxin-Mp2b recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyop
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyop
Sequence Positions
1-23aa, Full Length
Sequence
LIGLVSKGTCVLVKTVCKKVLKQ
Species
Myrmecia pilosula (Jack jumper ant) (Australian jumper ant)
Tag
N-terminal GST-tagged and C-terminal 6xHis-tagged
Function
Heterodimer protein that may serve both defensive (pain-inducing) and predatory (insecticidal) roles (PubMed:28513074). Has membrane-disrupting activity and shows induction of non-specific calcium influx into cells, (PubMed:28513074). Shows broad-spectrum activity against a diverse range of bacteria, and cell lines, as well as hemolytic activity (EC (50) =2.18uM) (PubMed:28513074). In vivo, shows moderate insecticidal activity against D.melanogaster and potent anthelmintic activity against the veterinary nematode H.contortus (PubMed:32629771). In addition, intraplantar injection into mice induces nocifensive behavior and mechanical allodynia (PubMed:28513074).
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
References
"It takes two: dimerization is essential for the broad-spectrum predatory and defensive activities of the venom peptide Mp1a from the jack jumper ant Myrmecia pilosula."Nixon S.A., Dekan Z., Robinson S.D., Guo S., Vetter I., Kotze A.C., Alewood P.F., King G.F., Herzig V.Biomedicines 8:0-0 (2020)
NCBI and Uniprot Product Information
Uniprot Description
Subunit structure: Heterodimer with pilosulin-3a (AC Q26464); disulfide-linked. This heterodimer is named pilosulin-3. Ref.1
Subcellular location: Secreted.
Tissue specificity: Expressed by the venom gland.
Sequence similarities: Belongs to the pilosulin family.
Mass spectrometry: Molecular mass is 2457 Da from positions 1 - 23. Determined by ESI. Ref.1
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Product Notes
The Myrmecia pilosula M-myrmeciitoxin-Mp2b (Catalog #AAA7137020) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-23aa, Full Length. The amino acid sequence is listed below: LIGLVSKGTC VLVKTVCKKV LKQ. It is sometimes possible for the material contained within the vial of "Myrmecia pilosula M-myrmeciitoxin-Mp2b, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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