E3 ubiquitin-protein ligase MYLIP Recombinant Protein | MYLIP recombinant protein

Recombinant Human E3 ubiquitin-protein ligase MYLIP

Cat. Num. AAA9421349

• Gene Names: MYLIP; MIR; IDOL
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase MYLIP; Recombinant Human E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; IdolMyosin regulatory light chain interacting protein; MIR; MYLIP recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-445aa

• Tag Info: GST-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for MYLIP recombinant protein

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.

NCBI and Uniprot Product Information

• NCBI GI #: 38788243
• NCBI GeneID: 29116
• NCBI Accession #: NP_037394.2
• NCBI GenBank Nucleotide #: NM_013262.3
• UniProt Accession #: Q8WY64; Q5TIA4; Q9BU73; Q9NRL9; Q9UHE7
• Molecular Weight: 77.3kD
• NCBI Official Full Name: E3 ubiquitin-protein ligase MYLIP
• NCBI Official Synonym Full Names: myosin regulatory light chain interacting protein
• NCBI Official Symbol: MYLIP
• NCBI Official Synonym Symbols: MIR; IDOL
• NCBI Protein Information: E3 ubiquitin-protein ligase MYLIP
• UniProt Protein Name: E3 ubiquitin-protein ligase MYLIP
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MYLIP
• UniProt Synonym Gene Names: BZF1; IDOL; Idol; MIR

NCBI Description

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

Uniprot Description

MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoskeleton; cytosol; intracellular

Molecular Function: cytoskeletal protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin-protein ligase activity

Biological Process: actomyosin structure organization and biogenesis; cell motility; low-density lipoprotein receptor catabolic process; nervous system development; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process