Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) Recombinant Protein | MUSK recombinant protein

Recombinant Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK)

Cat. Num. AAA2018741

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Muscle; Skeletal; Receptor Tyrosine Kinase (MUSK); Recombinant Muscle; MUSK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- CRRR KEWKNKKRES TAVTLTTLPS ELLLDRLHPN PMYQRMPLLL NPKLLSLEYP RNNIEYVRDI GEGAFGRVFQ ARAPGLLPYE PFTMVAVKML KEEASADMQA DFQREAALMA EFDNPNIVKL LGVCAVGKPM CLLFEYMAYG DLNEFLRSMS PHTVCSLSHS DLSTRARVSS PGPPPLSCAE QLCIARQVAA GMAYLSERKF VHRDLATRNC LVGETMVVKIADFGLSRNIY SADYYKADGN DAIPIRWMPP ESIFYNR
• Sequence Length: 878

• Applicable Applications for MUSK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Cys517~Arg777 (Accession # Q61006) with N-terminal His-Tag
• Subcellular Location: Cell junction, synapse, postsynaptic cell membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 82533041
• NCBI GeneID: 18198
• NCBI Accession #: NP_001032205.1
• NCBI GenBank Nucleotide #: NM_001037128.1
• UniProt Accession #: Q61006; Q61005; Q61987; Q61988
• Molecular Weight: 31.1kDa
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase; muscle localized kinase 2; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine protein kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal receptor tyrosine protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK
• UniProt Entry Name: MUSK_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; Musk family

Cellular Component: postsynaptic membrane; cell projection; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction; neuromuscular junction; receptor complex; external side of plasma membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; protein kinase binding; ATP binding; PDZ domain binding; protein kinase activity

Biological Process: positive regulation of synaptic transmission, cholinergic; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of synaptic growth at neuromuscular junction; signal transduction; protein amino acid phosphorylation; receptor clustering; memory; positive regulation of peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; response to electrical stimulus; cell differentiation; phosphorylation; neuromuscular junction development