Rat Mucin-7 ELISA Kit | MUC7 elisa kit

Rat Mucin-7 ELISA Kit

Cat. Num. AAA733793

• Gene Names: MUC7; MG2
• Reactivity: Rat
• Synonyms: Mucin-7; Rat Mucin-7 ELISA Kit; MUC7 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This MUC7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MUC7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC7 elisa kit

Principle of the Assay: MUC7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MUC7. Standards or samples are then added to the microtiter plate wells and MUC7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MUC7 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MUC7 are added to each well to "sandwich" the MUC7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MUC7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MUC7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 222418649
• NCBI GeneID: 4589
• NCBI Accession #: NP_001138479.1
• NCBI GenBank Nucleotide #: NM_001145007.1
• Molecular Weight: 39,159 Da
• NCBI Official Full Name: mucin-7
• NCBI Official Synonym Full Names: mucin 7, secreted
• NCBI Official Symbol: MUC7
• NCBI Official Synonym Symbols: MG2
• NCBI Protein Information: mucin-7; MUC-7; apo-MG2; salivary mucin-7; mucin 7, salivary
• UniProt Protein Name: Mucin-7
• Protein Family: Mucin
• UniProt Gene Name: MUC7
• UniProt Synonym Gene Names: MG2; MUC-7
• UniProt Entry Name: MUC7_HUMAN

NCBI Description

This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.[provided by RefSeq, Feb 2009]

Uniprot Description

MUC7: May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili. Genetic variations in MUC7 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification

Disease: Asthma, Susceptibility To