Mucin 3B Recombinant Protein | MUC3B recombinant protein

Recombinant Mucin 3B, Cell Surface Associated (MUC3B)

Cat. Num. AAA2029966

• Gene Names: MUC3A; MUC3; MUC-3A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Mucin 3B; Recombinant Mucin 3B; Cell Surface Associated (MUC3B); MUC3B recombinant protein

• Host: Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.!!Predicted isoelectric point|| 4.4!!Predicted Molecular Mass||14.0kDa
• Sequence: The sequence of the target protein is listed below.; TSVAT TQTPTTLTTC RTTPITSWMT TQSTLTTTAG TCDNGGTWEQ GQCACLPGFS GDRCQLQTRC QNGGQWDGLK CQCPSTFYGS SCEFAVEQVD L
• Sequence Length: 76

• Applicable Applications for MUC3B recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 14.0KDa
• Accurate Molecular Mass (KD): 14kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Accession: Q9H195
• Subcellular Location: Membrane; Single-pass membrane protein.
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Thr836~Leu931
• Usage: Reconstitute in sterile ddH2O.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 384381540
• NCBI GeneID: 4584
• NCBI Official Full Name: mucin 3B, partial
• NCBI Official Synonym Full Names: mucin 3A, cell surface associated
• NCBI Official Symbol: MUC3A
• NCBI Official Synonym Symbols: MUC3; MUC-3A
• NCBI Protein Information: mucin-3A
• UniProt Protein Name: Mucin-3A
• Protein Family: Mucin
• UniProt Gene Name: MUC3A
• UniProt Synonym Gene Names: MUC3; MUC-3A
• UniProt Entry Name: MUC3A_HUMAN

NCBI Description

The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 [PubMed 7778880]).[supplied by OMIM, Aug 2008]

Uniprot Description

MUC3A: Major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi lumen; integral to membrane; extracellular region

Molecular Function: extracellular matrix structural constituent; extracellular matrix constituent, lubricant activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification

Disease: Inflammatory Bowel Disease 11