Human Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 ELISA Kit | MTHFD2L elisa kit
Human Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 (MTHFD2L) ELISA Kit
Reactivity
Human
Synonyms
Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2; Human Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 (MTHFD2L) ELISA Kit; MTHFD2L elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MTHFD2L. No significant cross-reactivity or interference between Human MTHFD2L and analogues was observed.
Sequence Length
347
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/mL - 10 ng/mL
Sensitivity
0.078 ng/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MTHFD2L elisa kit
Intended Uses: For the quantitative detection of Human Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 (MTHFD2L) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MTHFD2L in Human serum, plasma, tissue homogenates. An antibody specific for MTHFD2L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTHFD2L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTHFD2L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTHFD2L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MTHFD2L in Human serum, plasma, tissue homogenates. An antibody specific for MTHFD2L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTHFD2L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTHFD2L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTHFD2L bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,891 Da
NCBI Official Full Name
probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2 isoform 2
NCBI Official Synonym Full Names
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like
NCBI Official Symbol
MTHFD2L
NCBI Protein Information
probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2
UniProt Protein Name
Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2
UniProt Gene Name
MTHFD2L
UniProt Synonym Gene Names
MTHFD2-like
Uniprot Description
MTHFD2L: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.5.1.15; EC 3.5.4.9; Hydrolase; Mitochondrial; Oxidoreductase
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion
Molecular Function: methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity
Biological Process: 10-formyltetrahydrofolate metabolic process; folic acid metabolic process; histidine biosynthetic process; methionine biosynthetic process; one-carbon metabolic process; purine nucleotide biosynthetic process
Research Articles on MTHFD2L
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Product Notes
The Human MTHFD2L mthfd2l (Catalog #AAA281821) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281821 ELISA Kit recognizes Human MTHFD2L. It is sometimes possible for the material contained within the vial of "Probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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