Human Metal regulatory transcription factor 1 ELISA Kit | MTF1 elisa kit

Human Metal regulatory transcription factor 1 ELISA Kit

Cat. Num. AAA763180

• Gene Names: MTF1; ZRF; MTF-1
• Reactivity: Human
• Synonyms: Metal regulatory transcription factor 1; Human Metal regulatory transcription factor 1 ELISA Kit; MTF1/MRE-binding transcription factor/Transcription factor MTF-1; MTF1 elisa kit

• Reactivity: Human
• Sequence Length: 753

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MTF1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MTF1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MTF1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MTF1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MTF1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 112790163
• NCBI GeneID: 4520
• NCBI Accession #: NP_005946.2
• NCBI GenBank Nucleotide #: NM_005955.2
• Molecular Weight: 80,957 Da
• NCBI Official Full Name: metal regulatory transcription factor 1
• NCBI Official Synonym Full Names: metal-regulatory transcription factor 1
• NCBI Official Symbol: MTF1
• NCBI Official Synonym Symbols: ZRF; MTF-1
• NCBI Protein Information: metal regulatory transcription factor 1
• UniProt Protein Name: Metal regulatory transcription factor 1
• Protein Family: Mitochondrial transcription factor
• UniProt Gene Name: MTF1
• UniProt Entry Name: MTF1_HUMAN

NCBI Description

This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008]

Uniprot Description

MTF1: Activates the metallothionein I promoter. Binds to the metal responsive element (MRE).

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: nucleoplasm; nucleus

Molecular Function: DNA binding; histone acetyltransferase binding; metal ion binding; protein binding; transcription coactivator activity; transcription factor activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to cadmium ion; response to metal ion; response to oxidative stress; transcription from RNA polymerase II promoter