Moesin Recombinant Protein | MSN recombinant protein

Moesin recombinant protein

Cat. Num. AAA515134

• Applications: Western Blot
• Synonyms: Moesin; Moesin recombinant protein; MSN; MSN recombinant protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 3981

• Applicable Applications for MSN recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant human Moesin (410-end) was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for MSN recombinant protein

Recombinant human Moesin (410-end) was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: Moesin (or membrane-organizing extension spike protein) belongs to ERM family that modulates epithelial integrity by regulating cell-signalling events that affect actin organization and polarity (1). The effects of Moesin on epithelial cells appear to result from inhibition of Rho signaling. ERM proteins serve a structural role in linkage of the cytoskeletion to the plasma membrane and the rescue of cells lacking Moesin by modulation of Rho signaling indicates that inhibition of Rho activity may be a more critical function of Moesin. The negative feedback loop produced by Rho's activation of ERM may be an important mechanism that prevents the excessive migratory and invasive properties characteristic of metastatic cancer cells (2).

Product Categories/Family for MSN recombinant protein

Proteins Substrates; Signaling Reagents - Protein Substrates

References

1. Speck, O. et al: Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity. Nature. 2003;421(6918):83-7.
2. Lankes, W.T. et akl: Moesin: a member of the protein 4.1-talin-ezrin family of proteins Proc. Natl. Acad. Sci. U S A. 1991;88(19):8297-301.

NCBI and Uniprot Product Information

• NCBI GI #: 53729335
• NCBI GeneID: 4478
• NCBI Accession #: NP_002435.1
• NCBI GenBank Nucleotide #: NM_002444
• UniProt Accession #: P26038
• Molecular Weight: ~50 kDa
• NCBI Official Full Name: Homo sapiens moesin (MSN), mRNA
• NCBI Official Synonym Full Names: moesin
• NCBI Official Symbol: MSN
• NCBI Protein Information: moesin; membrane-organizing extension spike protein
• UniProt Protein Name: Moesin
• Protein Family: Moesin
• UniProt Gene Name: MSN
• UniProt Entry Name: MOES_HUMAN

NCBI Description

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage. Ref.13

Enzyme regulation: A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding

By similarity.

Subunit structure: In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation

By similarity. Binds SLC9A3R1. Interacts with PPP1R16B. Interacts with PDZD8. Interacts with SELPLG and SYK; mediates the activation of SYK by SELPLG. Ref.5 Ref.6 Ref.7 Ref.13

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cytoplasm › cytoskeleton

By similarity. Apical cell membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cell projection › microvillus membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Note: Phosphorylated form is enriched in microvilli-like structures at apical membrane

By similarity. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. Ref.7

Tissue specificity: In all tissues and cultured cells studied.

Post-translational modification: Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding

By similarity. Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.

Sequence similarities: Contains 1 FERM domain.