Mouse mutS homolog 6 (E. coli) ELISA Kit | MSH6 elisa kit
Mouse DNA mismatch repair protein Msh6, MSH6 ELISA Kit
NCBI and Uniprot Product Information
Uniprot Description
MSH6: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Belongs to the DNA mismatch repair MutS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; plasma membrane; MutSalpha complex; intracellular; chromosome; nucleus
Molecular Function: DNA-dependent ATPase activity; single base insertion or deletion binding; single thymine insertion binding; oxidized purine DNA binding; ATPase activity; magnesium ion binding; nucleotide binding; ADP binding; methylated histone residue binding; mismatched DNA binding; four-way junction DNA binding; DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; damaged DNA binding; MutLalpha complex binding; chromatin binding; ATP binding
Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic hypermutation of immunoglobulin genes; isotype switching; somatic recombination of immunoglobulin gene segments; determination of adult life span; DNA repair; maintenance of DNA repeat elements; response to DNA damage stimulus; response to UV; meiotic mismatch repair
Research Articles on MSH6
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Product Notes
The Mouse MSH6 msh6 (Catalog #AAA9335010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9335010 ELISA Kit recognizes Mouse MSH6. It is sometimes possible for the material contained within the vial of "mutS homolog 6 (E. coli), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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