Rat mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) ELISA Kit | MSH2 elisa kit

Rat DNA mismatch repair protein Msh2, MSH2 ELISA Kit

Cat. Num. AAA9321793

• Reactivity: Rat
• Synonyms: mutS homolog 2; colon cancer; nonpolyposis type 1 (E. coli); Rat DNA mismatch repair protein Msh2; MSH2 ELISA Kit; Rat DNA mismatch repair protein Msh2 (MSH2) ELISA kit; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2 elisa kit

• Reactivity: Rat

• Preparation and Storage: Store all reagents at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 13591999
• NCBI GeneID: 81709
• NCBI Accession #: NP_112320.1
• NCBI GenBank Nucleotide #: NM_031058.1
• UniProt Accession #: P54275
• Molecular Weight: 104,028 Da
• NCBI Official Full Name: DNA mismatch repair protein Msh2
• NCBI Official Synonym Full Names: mutS homolog 2
• NCBI Official Symbol: Msh2
• NCBI Protein Information: DNA mismatch repair protein Msh2; mutS protein homolog 2
• UniProt Protein Name: DNA mismatch repair protein Msh2
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: Msh2
• UniProt Entry Name: MSH2_RAT

NCBI Description

mismatch repair protein; may have an important role in spermatogenesis [RGD, Feb 2006]

Uniprot Description

MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family.

Protein type: Tumor suppressor; DNA-binding

Cellular Component: nuclear chromosome; membrane; MutSalpha complex; intracellular; nucleus; MutSbeta complex

Molecular Function: protein homodimerization activity; dinucleotide repeat insertion binding; single thymine insertion binding; ATPase activity; magnesium ion binding; loop DNA binding; Y-form DNA binding; enzyme binding; four-way junction DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; ATP binding; single-stranded DNA binding; protein C-terminus binding; DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; single base insertion or deletion binding; oxidized purine DNA binding; ADP binding; protein kinase binding; mismatched DNA binding; centromeric DNA binding; DNA binding; damaged DNA binding; MutLalpha complex binding; dinucleotide insertion or deletion binding

Biological Process: somatic recombination of immunoglobulin genes during immune response; determination of adult life span; response to organic cyclic substance; maintenance of DNA repeat elements; germ cell development; positive regulation of helicase activity; double-strand break repair; negative regulation of neuron apoptosis; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; oxidative phosphorylation; response to X-ray; response to drug; negative regulation of DNA recombination; mismatch repair; in utero embryonic development; postreplication repair; male gonad development; somatic hypermutation of immunoglobulin genes; isotype switching; response to amino acid stimulus; DNA repair; meiotic mismatch repair; response to UV-B; B cell mediated immunity; DNA damage response, signal transduction resulting in induction of apoptosis; intra-S DNA damage checkpoint; B cell differentiation; somatic recombination of immunoglobulin gene segments; meiotic gene conversion; spermatogenesis; response to DNA damage stimulus; negative regulation of meiotic recombination