Canine Melanocyte Stimulating Hormone ELISA Kit | MSH elisa kit

Canine Melanocyte Stimulating Hormone ELISA Kit

Cat. Num. AAA24724

• Gene Names: MC1R; CMM5; MSH-R; SHEP2
• Reactivity: Canine
• Synonyms: Melanocyte Stimulating Hormone; Canine Melanocyte Stimulating Hormone ELISA Kit; MSH elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between Canine MSH and analogues was observed.
• Sequence Length: 317

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml - 100 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MSH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine MSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MSH and MSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MSH elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MSH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine MSH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MSH and MSH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119587080
• NCBI GeneID: 4157
• UniProt Accession #: Q01726; Q66K38; Q6UR93; Q8WWX6; Q8WWX7; Q96I33; Q96RU4; Q9UBF7; Q9UN58; Q9UN59; Q9UN60; Q9UN61
• Molecular Weight: 34,706 Da
• NCBI Official Full Name: melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
• NCBI Official Synonym Full Names: melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
• NCBI Official Symbol: MC1R
• NCBI Official Synonym Symbols: CMM5; MSH-R; SHEP2
• NCBI Protein Information: melanocyte-stimulating hormone receptor; MC1-R; melanotropin receptor
• UniProt Protein Name: Melanocyte-stimulating hormone receptor
• UniProt Gene Name: MC1R
• UniProt Synonym Gene Names: MSHR; MSH-R; MC1-R
• UniProt Entry Name: MSHR_HUMAN

NCBI Description

This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation. [provided by RefSeq, Jul 2008]

Uniprot Description

MC1R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5). Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; melanocortin receptor activity; hormone binding; melanocyte stimulating hormone receptor activity; ubiquitin protein ligase binding; peptide receptor activity, G-protein coupled

Biological Process: melanin biosynthetic process; positive regulation of protein kinase B signaling cascade; G-protein signaling, coupled to cyclic nucleotide second messenger; pigmentation; UV protection; multicellular organismal development; positive regulation of cAMP biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; sensory perception of pain

Disease: Albinism, Oculocutaneous, Type Ii; Increased Analgesia From Kappa-opioid Receptor Agonist, Female-specific; Melanoma, Cutaneous Malignant, Susceptibility To, 5; Skin/hair/eye Pigmentation, Variation In, 2