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SDS-Page

RNMTL1 recombinant protein

RNMTL1, 1-420aa, Human, His tag, E Coli (Denatured)

Gene Names
MRM3; RMTL1; RNMTL1
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
RNMTL1; 1-420aa; Human; His tag; E Coli (Denatured); RNA methyltransferase-like protein 1; HC90; RNMTL1 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid, In 20mM Tris-HCl (pH 8.0) containing 10% glycerol
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAALVRP ARFVVRPLLQ VVQAWDLDAR RWVRALRRSP VKVVFPSGEV VEQKRAPGKQ PRKAPSEASA QEQREKQPLE ESASRAPSTW EESGLRYDKA YPGDRRLSSV MTIVKSRPFR EKQGKILLEG RRLISDALKA GAVPKMFFFS RLEYLKELPV DKLKGVSLIK VKFEDIKDWS DLVTPQGIMG IFAKPDHVKM TYPKTQLQHS LPLLLICDNL RDPGNLGTIL RSAAGAGCSK VLLTKGCVDA WEPKVLRAGM GAHFRMPIIN NLEWETVPNY LPPDTRVYVA DNCGLYAQAE MSNKASDHGW VCDQRVMKFH KYEEEEDVET GASQDWLPHV EVQSYDSDWT EAPAAVVIGG ETYGVSLESL QLAESTGGKR LLIPVVPGVD SLNSAMAASI LLFEGKRQLR GRAEDLSRDR SYH
Sequence Length
420
Applicable Applications for RNMTL1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for RNMTL1 recombinant protein
RNMTL1 also known as RNA methyltransferase-like protein 1 is belong to the RNA methyltransferase TrmH family. This protein is probable RNA methyltransferase and expressed at same level in normal liver and hepatocarcinoma. Recombinant human RNMTL1, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for RNMTL1 recombinant protein
References
Xu J.,et al. (2002) Cell Res. 12:177-197

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49.4kDa (443aa)
NCBI Official Full Name
rRNA methyltransferase 3, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial rRNA methyltransferase 3
NCBI Official Symbol
MRM3
NCBI Official Synonym Symbols
RMTL1; RNMTL1
NCBI Protein Information
rRNA methyltransferase 3, mitochondrial
UniProt Protein Name
rRNA methyltransferase 3, mitochondrial
UniProt Gene Name
MRM3

NCBI Description

Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.

Research Articles on RNMTL1

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Product Notes

The RNMTL1 mrm3 (Catalog #AAA206091) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RNMTL1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RNMTL1 mrm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAALVRP ARFVVRPLLQ VVQAWDLDAR RWVRALRRSP VKVVFPSGEV VEQKRAPGKQ PRKAPSEASA QEQREKQPLE ESASRAPSTW EESGLRYDKA YPGDRRLSSV MTIVKSRPFR EKQGKILLEG RRLISDALKA GAVPKMFFFS RLEYLKELPV DKLKGVSLIK VKFEDIKDWS DLVTPQGIMG IFAKPDHVKM TYPKTQLQHS LPLLLICDNL RDPGNLGTIL RSAAGAGCSK VLLTKGCVDA WEPKVLRAGM GAHFRMPIIN NLEWETVPNY LPPDTRVYVA DNCGLYAQAE MSNKASDHGW VCDQRVMKFH KYEEEEDVET GASQDWLPHV EVQSYDSDWT EAPAAVVIGG ETYGVSLESL QLAESTGGKR LLIPVVPGVD SLNSAMAASI LLFEGKRQLR GRAEDLSRDR SYH. It is sometimes possible for the material contained within the vial of "RNMTL1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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