Meiotic Recombination 11 Homolog A Recombinant Protein | MRE11A recombinant protein

Recombinant Meiotic Recombination 11 Homolog A (MRE11A)

Cat. Num. AAA2029552

• Gene Names: MRE11A; ATLD; HNGS1; MRE11; MRE11B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Meiotic Recombination 11 Homolog A; Recombinant Meiotic Recombination 11 Homolog A (MRE11A); MRE11A recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; PFSVLR FSQKFVDRVA NPKDIIHFFRHREQKEKTGE EINFGKLITK PSEGTTLRVE DLVKQYFQTA EKNVQLSLLTERGMGEAVQE FVDKEEKDAI EELVKYQLEK TQRFLKERHI DALEDKIDEEVRRFRETRQK NTNEEDDEVR EAMTRARALR SQSEESASAF SADDLMSIDLAEQMANDSDD SISAATNKGR GRGRGRRGGR GQNSASRGGS QRGRADTGLETSTRSRNSK
• Sequence Length: 680

• Applicable Applications for MRE11A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 56.8kDa
• Accurate Molecular Mass (KD): 59kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro375~Lys609
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119587338
• NCBI GeneID: 4361
• Molecular Weight: 81,070 Da
• NCBI Official Full Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae), isoform CRA_c
• NCBI Official Synonym Full Names: MRE11 homolog A, double strand break repair nuclease
• NCBI Official Symbol: MRE11A
• NCBI Official Synonym Symbols: ATLD; HNGS1; MRE11; MRE11B
• NCBI Protein Information: double-strand break repair protein MRE11A
• UniProt Protein Name: Double-strand break repair protein MRE11A
• Protein Family: Double-strand break repair protein
• UniProt Gene Name: MRE11A
• UniProt Synonym Gene Names: HNGS1; MRE11; MRE11 homolog 1; MRE11 homolog A
• UniProt Entry Name: MRE11_HUMAN

NCBI Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

MRE11A: a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. Alternative splicing results in two different isoforms.

Protein type: DNA repair, damage; Deoxyribonuclease; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: nucleoplasm; chromosome, telomeric region; Mre11 complex; chromatin; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; nuclease activity; DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; manganese ion binding; endonuclease activity; double-stranded DNA binding; 3'-5' exonuclease activity; endodeoxyribonuclease activity

Biological Process: positive regulation of kinase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; telomere maintenance via telomerase; regulation of mitotic recombination; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; intra-S DNA damage checkpoint; nucleotide-excision repair; base-excision repair; meiotic recombination; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; synapsis; positive regulation of interferon type I production; innate immune response; response to DNA damage stimulus; telomere maintenance

Disease: Ataxia-telangiectasia-like Disorder 1