MPP5 recombinant protein

Recombinant Human MPP5 protein

Cat. Num. AAA1561452

• Gene Names: MPP5; PALS1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MPP5; Recombinant Human MPP5 protein; MAGUK p55 subfamily member 5; MPP5 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human MPP5 (Asn455-Arg676) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for MPP5 recombinant protein

Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface. Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity. Required during embryonic and postnatal retinal development. Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation and cerebellar layer organization. Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Plays a role in the T-cell receptor-mediated activation of NF-kappa-B. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. Required for the normal polarized localization of the vesicular marker STX4. Required for the correct trafficking of the myelin proteins PMP22 and MAG.

Product Categories/Family for MPP5 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 116242632
• NCBI GeneID: 64398
• UniProt Accession #: Q8N3R9; Q7Z631; Q86T98; Q8N7I5; Q9H9Q0; A1L380
• Molecular Weight: 675
• NCBI Official Full Name: MAGUK p55 subfamily member 5
• NCBI Official Synonym Full Names: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• NCBI Official Symbol: MPP5
• NCBI Official Synonym Symbols: PALS1
• NCBI Protein Information: MAGUK p55 subfamily member 5; stardust; protein associated with lin seven 1
• UniProt Protein Name: MAGUK p55 subfamily member 5
• Protein Family: MAGUK p55 subfamily
• UniProt Gene Name: MPP5
• UniProt Entry Name: MPP5_HUMAN

NCBI Description

This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MPP5: May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. Belongs to the MAGUK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: nucleoplasm; protein complex; tight junction; lateral loop; cytoplasm; plasma membrane; endomembrane system

Molecular Function: protein domain specific binding; protein binding

Biological Process: intercellular junction assembly and maintenance; myelin formation; myelin maintenance in the peripheral nervous system; morphogenesis of an epithelial sheet