Mouse anti-Human Ku80 Antigen Antibody | anti-XRCC5 antibody

Anti-Ku80 Antigen Purified

Cat. Num. AAA1801229

• Gene Names: XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunoprecipitation
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: Ku80 Antigen; Anti-Ku80 Antigen Purified; X-ray repair cross complementing 5; DNA helicase p80; anti-XRCC5 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Clone Number: MEM-54
• Specificity: The antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa intracellular polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 732

• Applicable Applications for anti-XRCC5 antibody: Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen: human whole T-lymphocytes
• Preparation and Storage: Store at 2-8 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa human cervix carcinoma cell line using purified anti-Ku Antigen (MEM-54) (detection by Goat anti-mouse IgG2a Alexa Fluor 488; green). Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).)

Related Product Information for anti-XRCC5 antibody

Ku Antigen (DNA-dependent DNA helicase) is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.

NCBI and Uniprot Product Information

• NCBI GI #: 10863945
• NCBI GeneID: 7520
• NCBI Accession #: NP_066964.1
• NCBI GenBank Nucleotide #: NP_066964.1
• UniProt Accession #: P13010
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair cross complementing 5
• NCBI Official Symbol: XRCC5
• NCBI Official Synonym Symbols: KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• NCBI Protein Information: X-ray repair cross-complementing protein 5
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: XRCC5
• UniProt Synonym Gene Names: G22P2; CTC85; CTCBF; TLAA
• UniProt Entry Name: XRCC5_HUMAN

NCBI Description

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: RNA-binding; DNA-binding; EC 3.6.4.-; Nuclear receptor co-regulator; Helicase; Nucleolus; EC 3.6.1.-

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nuclear telomere cap complex; membrane; nuclear chromosome, telomeric region; nucleolus; plasma membrane; nucleus; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: response to drug; cell proliferation; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; DNA repair; negative regulation of transcription, DNA-dependent; telomere maintenance; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA recombination