ARHGAP35 sirna

ARHGAP35 siRNA (Mouse)

Cat. Num. AAA8229396

• Gene Names: Arhgap35; Grlf1; p190A; AI841135; mKIAA1722; p190RhoGAP; 6430596G11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARHGAP35; ARHGAP35 siRNA (Mouse); GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; ARHGAP35 sirna

• Reactivity: Mouse
• Specificity: ARHGAP35 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1499

• Applicable Applications for ARHGAP35 sirna: RNA Interference (RNAi)
• Source: Synthetic
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARHGAP35 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARHGAP35 sirna

siRNA to inhibit ARHGAP35 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 75677442
• NCBI GeneID: 232906
• NCBI Accession #: NP_766327.3
• NCBI GenBank Nucleotide #: NM_172739.4
• UniProt Accession #: Q91YM2; Q3UGY1; Q69ZC4
• Molecular Weight: 170,393 Da
• NCBI Official Full Name: rho GTPase-activating protein 35
• NCBI Official Synonym Full Names: Rho GTPase activating protein 35
• NCBI Official Symbol: Arhgap35
• NCBI Official Synonym Symbols: Grlf1; p190A; AI841135; mKIAA1722; p190RhoGAP; 6430596G11Rik
• NCBI Protein Information: rho GTPase-activating protein 35
• UniProt Protein Name: Rho GTPase-activating protein 35
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: Arhgap35
• UniProt Synonym Gene Names: Grlf1; Kiaa1722
• UniProt Entry Name: RHG35_MOUSE

Uniprot Description

GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described.

Protein type: Tumor suppressor; GAPs, Rac/Rho; Transcription factor; Motility/polarity/chemotaxis; GAPs

Cellular Component: cytoplasm; intracellular; nucleus; actin cytoskeleton

Molecular Function: GTP binding; DNA binding; GTPase activator activity

Biological Process: integrin-mediated signaling pathway; camera-type eye development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of cell shape; negative regulation of Rho protein signal transduction; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; forebrain development; neural tube closure; negative regulation of vascular permeability; negative regulation of transcription, DNA-dependent; positive regulation of GTPase activity