Mouse Tumor Protein P63 (TP63) RTU ELISA Kit | TP63 rtu elisa kit

Mouse Tumor Protein P63 (TP63) ELISA Kit

Cat. Num. AAA4503052

• Gene Names: Trp63; Ket; P63; P73l; Tp63; P51/P63; AI462811; Trp53rp1
• Reactivity: Mouse
• Synonyms: Tumor Protein P63 (TP63); Mouse Tumor Protein P63 (TP63) ELISA Kit; P63; TP73L; CUSP; EEC3; KET; LMS; OFC8; RHS; SHFM4; TP53CP; Tumor Protein p53-Competing Protein; Chronic ulcerative stomatitis protein; Keratinocyte transcription factor; TP63 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 680

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP63 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TP63. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TP63. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TP63, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TP63 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 189083804
• NCBI GeneID: 22061
• NCBI Accession #: NP_001120731.1
• NCBI GenBank Nucleotide #: NM_001127259.1
• UniProt Accession #: O88898; O88897; O88899; O89097; Q8C826; Q9QWY9; Q9QWZ0
• Molecular Weight: 43,970 Da
• NCBI Official Full Name: tumor protein 63 isoform a
• NCBI Official Synonym Full Names: transformation related protein 63
• NCBI Official Symbol: Trp63
• NCBI Official Synonym Symbols: Ket; P63; P73l; Tp63; P51/P63; AI462811; Trp53rp1
• NCBI Protein Information: tumor protein 63
• UniProt Protein Name: Tumor protein 63
• Protein Family: Tumor protein
• UniProt Gene Name: Tp63
• UniProt Synonym Gene Names: P63; P73l; Tp73l; Trp63; p63; TP63; p73L

NCBI Description

This gene encodes tumor protein p63, a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include tumor proteins p53, p63, and p73, which have high sequence similarity to one another. This similarity allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways, including direct and indirect protein interactions. This results in mutual regulation of target gene promoters. Tumor protein p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Both alternative splicing and the use of alternative promoters result in multiple transcript variants encoding different protein isoforms.[provided by RefSeq, Dec 2009]

Uniprot Description

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Activates transcription of the p21 promoter (). Activates RIPK4 transcription. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge.