Mouse Secretin (SCT) RTU ELISA Kit | SCT rtu elisa kit

Mouse Secretin (SCT) ELISA Kit

Cat. Num. AAA4502390

• Reactivity: Mouse
• Synonyms: Secretin (SCT); Mouse Secretin (SCT) ELISA Kit; SCT rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 133

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 23.4-1500pg/mL
• Sensitivity: 9.46pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Related Product Information for SCT rtu elisa kit

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to SCT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled SCT and unlabeled SCT (Standards or samples) with the pre-coated antibody specific to SCT. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of SCT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of SCT in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 6755416
• NCBI GeneID: 20287
• NCBI Accession #: NP_035458.1
• NCBI GenBank Nucleotide #: NM_011328.3
• UniProt Accession #: Q08535
• Molecular Weight: 14,914 Da
• NCBI Official Full Name: secretin isoform 2 preproprotein
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: Sct
• NCBI Protein Information: secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: Sct

NCBI Description

This gene encodes the precursor of a gastrointestinal peptide hormone of the secretin-glucagon family. The encoded protein is secreted as a prohormone that undergoes proteolytic processing to generate a mature peptide hormone. The mature peptide regulates secretion of gastric acid, biocarbonate ions from pancreatic and biliary duct epithelia and water homeostasis in the gastrointestinal system. Mice lacking the encoded protein display decreased survival of neuroprogenitor cells during early postnatal period and impaired long-term potentiation and spatial learning in adulthood. Alternative splicing results in multiple transcript variants encoding different isoforms. All of these isoforms may be processed in a similar manner to generate the mature peptide hormone. [provided by RefSeq, Jul 2015]

Uniprot Description

Stimulates formation of NaHCO3-rich pancreatic juice and secretion of NaHCO3-rich bile and inhibits HCl production by the stomach.