Mouse Histidine Rich Glycoprotein (HRG) RTU ELISA Kit | HRG rtu elisa kit

Mouse Histidine Rich Glycoprotein (HRG) ELISA Kit

Cat. Num. AAA459912

• Gene Names: Hrg; Hprg; Hrgp; D16jh2; D18020; AI265597; AW413091
• Reactivity: Mouse
• Synonyms: Histidine Rich Glycoprotein (HRG); Mouse Histidine Rich Glycoprotein (HRG) ELISA Kit; HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG; HRG rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 536

• Assay Type: Sandwich
• Samples: Plasma.
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.28ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HRG rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HRG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HRG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HRG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HRG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226958456
• NCBI GeneID: 94175
• NCBI Accession #: NP_444406.2
• NCBI GenBank Nucleotide #: NM_053176.2
• UniProt Accession #: Q9ESB3; Q6YK32; Q6YKA2; Q99PS5; Q99PS6
• Molecular Weight: 59,163 Da
• NCBI Official Full Name: histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine-rich glycoprotein
• NCBI Official Symbol: Hrg
• NCBI Official Synonym Symbols: Hprg; Hrgp; D16jh2; D18020; AI265597; AW413091
• NCBI Protein Information: histidine-rich glycoprotein
• UniProt Protein Name: Histidine-rich glycoprotein
• UniProt Gene Name: Hrg
• UniProt Synonym Gene Names: HPRG

Uniprot Description

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis (). Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2.