Protein G Reagent
Protein G (Agarose), Fast Flow
Reactivity
Mouse, Rabbit, Sheep
Applications
Immunoprecipitation
Synonyms
Protein G; Protein G (Agarose); Fast Flow; Protein G reagent
Reactivity
Mouse, Rabbit, Sheep
Form/Format
Supplied as 10ml packed beads, containing 2mg/ml Protein G suspended as a 50% slurry in distilled water containing 20% ethanol for a final volume of 20ml. Binding capacity of 20mg human IgG/ml agarose. Prior to use, wash the agarose beads with an appropri
Applicable Applications for Protein G reagent
Immunoprecipitation (IP)
Application Notes
Suitable for Immunoprecipitation.
Dilution: Antibody Purification: A mouse monoclonal IgG was purified from hybridoma supernatant fraction using a 12ml column of Protein G (Agarose), Fast Flow and a Waters 650E Advanced Protein Purification System.
Elution Profile and Analysis: Mouse IgG was purified from 2L of hybridoma supernatant fraction using Protein G Agarose, Fast Flow. The column eluent was monitored with an UV detector and recorded at 280nm.
Immunoprecipitation: 20ul of a 50% slurry (10ul packed beads) was used to capture a mouse IgG Immunoprecipitation complex. Immunoprecipitation of Phosphotyrosine containing proteins contained in EGFstimulated A431 cell lysate using anti-phosphotyrosine, (4ug) in conjunction with 20ul (50% slurry) Protein G Agarose, fast flow followed by immunoblot analysis using antiphosphotyrosine. Blot was developed using an HRP-conjugated secondary antibody and the ECL detection system.
Dilution: Antibody Purification: A mouse monoclonal IgG was purified from hybridoma supernatant fraction using a 12ml column of Protein G (Agarose), Fast Flow and a Waters 650E Advanced Protein Purification System.
Elution Profile and Analysis: Mouse IgG was purified from 2L of hybridoma supernatant fraction using Protein G Agarose, Fast Flow. The column eluent was monitored with an UV detector and recorded at 280nm.
Immunoprecipitation: 20ul of a 50% slurry (10ul packed beads) was used to capture a mouse IgG Immunoprecipitation complex. Immunoprecipitation of Phosphotyrosine containing proteins contained in EGFstimulated A431 cell lysate using anti-phosphotyrosine, (4ug) in conjunction with 20ul (50% slurry) Protein G Agarose, fast flow followed by immunoblot analysis using antiphosphotyrosine. Blot was developed using an HRP-conjugated secondary antibody and the ECL detection system.
Preparation and Storage
4 degree C
Related Product Information for Protein G reagent
Stable in all aqueous buffers used in Protein G chromatography. Maximum linear flow rate 1300cm/hr. Recommended for flow rates from 30 to 400cm/hr. Useful for purifying IgG from mouse, sheep and rabbit.
Product Categories/Family for Protein G reagent
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Protein G (Catalog #AAA635334) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. The Protein G (Agarose), Fast Flow reacts with Mouse, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Protein G can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Suitable for Immunoprecipitation. Dilution: Antibody Purification: A mouse monoclonal IgG was purified from hybridoma supernatant fraction using a 12ml column of Protein G (Agarose), Fast Flow and a Waters 650E Advanced Protein Purification System. Elution Profile and Analysis: Mouse IgG was purified from 2L of hybridoma supernatant fraction using Protein G Agarose, Fast Flow. The column eluent was monitored with an UV detector and recorded at 280nm. Immunoprecipitation: 20ul of a 50% slurry (10ul packed beads) was used to capture a mouse IgG Immunoprecipitation complex. Immunoprecipitation of Phosphotyrosine containing proteins contained in EGFstimulated A431 cell lysate using anti-phosphotyrosine, (4ug) in conjunction with 20ul (50% slurry) Protein G Agarose, fast flow followed by immunoblot analysis using antiphosphotyrosine. Blot was developed using an HRP-conjugated secondary antibody and the ECL detection system. Researchers should empirically determine the suitability of the Protein G for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein G, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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