Interleukin 9, Rat Active Protein | IL9 active protein

Interleukin 9, Recombinant, Rat (IL-9)

Cat. Num. AAA650307

• Gene Names: IL9; P40; HP40; IL-9
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Interleukin 9; Rat; Recombinant; Rat (IL-9); IL9 active protein

• Host: Mouse myeloma cell line, NS0
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 30% CH3CN, 0.1% TFA, with 50ug BSA per 1ug of cytokine. Reconstitute with PBS, 0.1% HSA or BSA to a concentration of 10ug/ml.

• Biological Activity: Measured in a cell proliferation assay using a factor-dependent mouse cell line, TS1.
• Endotoxin Level: < 1.0 EU per 1ug of the protein as determined by the LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding PBS containing 0.1% HSA or BSA. Reconstituted product is stable for 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IL9 active protein

Rat interleukin-9 (IL-9; also T-cell growth factor P40 and MEA) is a 20-30 kD secreted glycoprotein that belongs to the IL-7/IL-9 family of hematopoietic cytokines. It is best considered an atypical Th2-type cytokine. 1-4 Rat IL-9 was initially isolated from the 4437A rat thymoma cell line. 5 The IL-9 precursor is 144 amino acids (aa) in length and contains an 18 aa signal sequence plus a 126 aa mature segment. 5 An extended, 219 aa alternate precursor form is reported in GenBank (Accession # XP_001068265). It shows a 113 aa substitution for the first 38 aa of the standard form. Its significance is unknown. Mature rat IL-9 is 75%, 74% and 58% aa identical to cotton rat, mouse and human IL-9, respectively. 6-9 There is variability in cross-species reactivity. Canine and mouse IL-9 are purportedly active on human cells, 9-11 while human IL-9 is inactive on mouse cells. 9 Cells reported to express IL-9 include activated CD4+ T cells and eosinophils. 12, 13 The receptor for rat IL-9 is composed of the 62 kD rat IL-9Ra chain and the 64 kD rat common cytokine receptor g-chain. 5, 14, 15 IL-9 is suggested to play a significant, but likely supporting role in allergy and asthma. Its synthesis is induced upon exposure to allergen. 10, 13 Following release, it is known to upregulate IgE production by IL-4-stimulated B cells. 16 It also stimulates mucin secretion and IL-13 production through a direct action on respiratory epithelium. 10, 17 In the bone marrow, it promotes eosinophilia, likely through IL-5 induction. 18, 19 And once produced, eosinophils are chemoattracted to the bronchi through the IL9-induced secretion of eotaxin-1 by airway smooth muscle cells.

Product Categories/Family for IL9 active protein

Growth Factors; Cytokines; Growth Factors-Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NM_000590.1
• UniProt Accession #: P15248; Q6NZ74; Q6NZ75
• Molecular Weight: The mature recombinant rat IL-9, generated by proteolytic removal of the signal peptide region, is a monomer protein. Based on N-terminal amino acid sequencing, the recombinant rat IL-9 starts at Gln 19. It has a calculated molecular mass of 14.1 kD. As a
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9; cytokine P40; p40 cytokine; OTTHUMP00000159423; T-cell growth factor p40; p40 T-cell and mast cell growth factor; homolog of mouse T cell and mast cell growth factor 40
• UniProt Protein Name: Interleukin-9
• Protein Family: Interleukin
• UniProt Gene Name: IL9
• UniProt Entry Name: IL9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq]

Uniprot Description

Function: Supports IL-2 independent and IL-4 independent growth of helper T-cells.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-7/IL-9 family.