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Western Blot (WB) (Figure 1. Western blot analysis using TUG mouse mAb against NIH/3T3 cell lysate.)

Mouse anti-Mouse TUG Antibody | anti-TUG antibody

Mouse TUG Antibody

Gene Names
ASPSCR1; TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
Reactivity
Mouse
Applications
Western Blot, ELISA
Synonyms
TUG; Mouse TUG Antibody; ASPCR1; ASPL; ASPS; RCC17; UBXD9; UBXN9; anti-TUG antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse
Isotype
IgG1
Clone Number
4A11A6G11
Form/Format
Purified antibody in PBS containing 0.03% sodium azide.
Sequence Length
647
Applicable Applications for anti-TUG antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of TUG expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using TUG mouse mAb against NIH/3T3 cell lysate.)

Western Blot (WB) (Figure 1. Western blot analysis using TUG mouse mAb against NIH/3T3 cell lysate.)
Related Product Information for anti-TUG antibody
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,910 Da
NCBI Official Full Name
tether containing UBX domain for GLUT4 isoform 2
NCBI Official Synonym Full Names
ASPSCR1, UBX domain containing tether for SLC2A4
NCBI Official Symbol
ASPSCR1
NCBI Official Synonym Symbols
TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
NCBI Protein Information
tether containing UBX domain for GLUT4
UniProt Protein Name
Tether containing UBX domain for GLUT4
UniProt Gene Name
ASPSCR1
UniProt Synonym Gene Names
ASPL; RCC17; TUG; UBXD9; UBXN9

NCBI Description

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]

Uniprot Description

Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (). Enhances VCP methylation catalyzed by VCPKMT.

Research Articles on TUG

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Product Notes

The TUG aspscr1 (Catalog #AAA850456) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse TUG Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TUG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the TUG aspscr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUG, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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