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Western Blot (WB) (Figure 1. Western blot analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.)

Mouse anti-Human, Mouse AMACR Antibody | anti-AMACR antibody

AMACR Antibody

Gene Names
AMACR; RM; RACE; CBAS4; P504S; AMACRD
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
AMACR; AMACR Antibody; RACE; anti-AMACR antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2b
Clone Number
2A10F3
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
394
Applicable Applications for anti-AMACR antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human AMACR expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.)

Western Blot (WB) (Figure 1. Western blot analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Figure 3. Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR mouse mAb.)

Immunohistochemistry (IHC) (Figure 3. Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR mouse mAb.)
Related Product Information for anti-AMACR antibody
AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate. high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42kDa
NCBI Official Full Name
alpha-methylacyl-CoA racemase isoform 3
NCBI Official Synonym Full Names
alpha-methylacyl-CoA racemase
NCBI Official Symbol
AMACR
NCBI Official Synonym Symbols
RM; RACE; CBAS4; P504S; AMACRD
NCBI Protein Information
alpha-methylacyl-CoA racemase
UniProt Protein Name
Alpha-methylacyl-CoA racemase
UniProt Gene Name
AMACR

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.

Research Articles on AMACR

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Product Notes

The AMACR amacr (Catalog #AAA850287) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AMACR Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMACR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the AMACR amacr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMACR, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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