Mouse Granulocyte-Colony Stimulating Factor (G-CSF) Antibody | anti-G-CSF antibody

Granulocyte-Colony Stimulating Factor (G-CSF)

Cat. Num. AAA4380815

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Reactivity: Human, Macaque Monkey
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Granulocyte-Colony Stimulating Factor (G-CSF); Pluripoietin; Filgrastim; Lenograstim; CSF3OS; G-CSF; chromosome 17 open reading frame 33 (C17orf33); colony stimulating factor 3 (granulocyte); anti-G-CSF antibody

• Host: Mouse
• Reactivity: Human, Macaque Monkey
• Isotype: IgG1, kappa
• Clone Number: rCSF3/900
• Specificity: This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 168

• Applicable Applications for anti-G-CSF antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Tonsil or lymph node (IHC).
• Immunogen: Recombinant full-length human CSF3 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 17q21.1
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with G-CSF Recombinant Mouse Monoclonal Antibody (rCSF3/900).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with G-CSF Recombinant Mouse Monoclonal Antibody (rCSF3/900).)

SDS-Page

(SDS-PAGE Analysis Purified G-CSF Recombinant Mouse Monoclonal Antibody (rCSF3/900). Confirmation of Purity and Integrity of Antibody.)

References

Nagata, S., et al. 1986. Nature 319: 415-418.

NCBI and Uniprot Product Information

• NCBI GI #: 296011057
• NCBI GeneID: 1440
• NCBI Accession #: NP_001171618.1
• NCBI GenBank Nucleotide #: NP_001171618.1
• UniProt Accession #: P09919
• Molecular Weight: 19kDa
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform d
• NCBI Official Synonym Full Names: colony stimulating factor 3
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell cycle regulation; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: granulocyte differentiation; positive regulation of myeloid cell differentiation; positive regulation of protein binding; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter