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Western Blot (WB) (Figure 1. Western blot analysis using KLK3 mouse mAb against truncated KLK3 recombinant protein.)

Mouse anti-Human PSA Antibody | anti-PSA antibody

PSA (KLK3) Antibody

Gene Names
KLK3; APS; PSA; hK3; KLK2A1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Synonyms
PSA; PSA (KLK3) Antibody; APS; hK3; KLK2A1; KLK3; anti-PSA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
5A11E9; 5A11E2
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
238
Applicable Applications for anti-PSA antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Purified recombinant fragment of KLK3 (aa26-251) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1. Western blot analysis using KLK3 mouse mAb against truncated KLK3 recombinant protein.)

Western Blot (WB) (Figure 1. Western blot analysis using KLK3 mouse mAb against truncated KLK3 recombinant protein.)

Immunohistochemistry (IHC)

(Figure 2. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using KLK3 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissues, showing cytoplasmic localization using KLK3 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Figure 3. Immunohistochemical analysis of paraffin-embedded human prostate tissues using KLK3 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 3. Immunohistochemical analysis of paraffin-embedded human prostate tissues using KLK3 mouse mAb with DAB staining.)
Related Product Information for anti-PSA antibody
Kallikrein-related peptidase 3.Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the identification and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
354
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,116 Da
NCBI Official Full Name
prostate-specific antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
kallikrein related peptidase 3
NCBI Official Symbol
KLK3
NCBI Official Synonym Symbols
APS; PSA; hK3; KLK2A1
NCBI Protein Information
prostate-specific antigen
UniProt Protein Name
Prostate-specific antigen
Protein Family
UniProt Gene Name
KLK3
UniProt Synonym Gene Names
APS; PSA; Seminin

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Research Articles on PSA

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Product Notes

The PSA klk3 (Catalog #AAA850995) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PSA (KLK3) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the PSA klk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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