Immunohistochemistry (IHC)
(Figure 1: Strong nuclear staining of p53 (DO-1) in a formalin-fixed, paraffin-embedded human colon carcinoma.)
Mouse anti-Human P53 Antibody
Mouse anti P53 (TP53 tumor suppressor protein)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunoblot, Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Synonyms
P53; Mouse anti P53 (TP53 tumor suppressor protein); TP53 tumor suppressor protein; anti-P53 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a/kappa
Clone Number
DO-1
Specificity
DO-1 recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (amino acids 11-25) of p53.
Form/Format
Each vial contains 100ul 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.05% sodium azide
Applicable Applications for anti-P53 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunoblot (IB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)-Frozen
Application Notes
The DO-1 antibody is suitable for the detection of p53 by Western blotting and detects a band of 53kDa in tumor tissue and cell extracts. The antibody is suitable for immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The antibody can also be applied in ELISA for the quantification of the p53 protein. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range is 1:500 – 1:1000 for flow cytometry, 1:500-1:2000 for immunohistochemistry, and 1:500 – 1:1000 for immunoblotting applications.
Source Note
DO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.
Preparation and Storage
Store at 4 degree C or for longer periods at -20 degree C in small aliquots.
Immunohistochemistry (IHC)
(Figure 1: Strong nuclear staining of p53 (DO-1) in a formalin-fixed, paraffin-embedded human colon carcinoma.)
Immunohistochemistry (IHC)
(Figure 1: Strong nuclear staining of p53 (DO-1) in a formalin-fixed, paraffin-embedded human colon carcinoma.)
Related Product Information for anti-P53 antibody
P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Alterations of the p53 tumor suppressor gene are considered critical events in multistage carcinogenesis of a wide range of human cancers. The mutations found in sporadic cancer are commonly missense mutations that inactivate the tumor suppressor activity of p53. These mutant proteins are much more stable than the normal p53 protein, frequently adopt an altered conformation and accumulate within the nucleus of the malignant cells. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Positive nuclear staining with p53 antibodies has been reported to be a negative prognostic factor in amongst others breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma.
Product Categories/Family for anti-P53 antibody
Similar Products
Product Notes
The P53 (Catalog #AAA573781) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti P53 (TP53 tumor suppressor protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P53 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunoblot (IB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)-Frozen . The DO-1 antibody is suitable for the detection of p53 by Western blotting and detects a band of 53kDa in tumor tissue and cell extracts. The antibody is suitable for immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The antibody can also be applied in ELISA for the quantification of the p53 protein. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range is 1:500 – 1:1000 for flow cytometry, 1:500-1:2000 for immunohistochemistry, and 1:500 – 1:1000 for immunoblotting applications. Researchers should empirically determine the suitability of the P53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P53, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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