Mouse anti-Human CD49d/VLA-4 Antibody
Mouse Anti-Human CD49d/VLA-4-UNLB
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
CD49d/VLA-4; Mouse Anti-Human CD49d/VLA-4-UNLB; anti-CD49d/VLA-4 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
7.2
Specificity
Human CD49d/VLA-4 CD49d, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha4 chain. CD49d associates noncovalently with CD29, the integrin beta1 subunit, to form the alpha4beta1 very late antigen-4 (VLA-4) heterodimer. Integrin alpha4 can also associate with integrin beta7 to form the alpha4beta7 heterodimer. It is expressed on most leukocytes, with the possible exception of neutrophils. VLA-4 mediates leukocyte binding to CD106/VCAM-1, and is involved in the migration of leukocytes from blood to tissue at sites of inflammation. VLA-4 can also provide a costimulatory signal to T cells for activation and proliferation. The alpha4beta7 integrin heterodimer is expressed on a small subpopulation of thymocytes, most lymph node T and B lymphocytes, NK cells and eosinophils. Integrin alpha4beta7 also binds to fibronectin and VCAM-1.1-4
Form/Format
Purified (UNLB) Antibody
Applicable Applications for anti-CD49d/VLA-4 antibody
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow cytometry, Immunoprecipitation, Western blotting
Flow Cytometry: <=1 ug/10^6 cells
Flow Cytometry: <=1 ug/10^6 cells
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The fluorescein (FITC), Alexa Fluor 488 (AF488), Alexa Fluor 647 (AF647), and Cyanine 5 (CY5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Product Categories/Family for anti-CD49d/VLA-4 antibody
References
1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD49d Section, Academic Press, New York, p. 262. 2. Lobb, R.R., and M.E. Hemler. 1994. J. Clin. Invest. 94:1722. 3. Alon, R., P.D. Kassner, M.W. Carr, E.B. Finger, M.E. Hemler, and T.A. Springer. 1995. J. Cell. Biol. 128:1243. 4. Berlin, C.,R.F. Bargatze, J.J. Campbell, U.H. von Andrian, M.C. Szabo, S.R. Hasslen, R.D. Nelson, E.L. Berg, S.L. Erlandsen, and E.C. Butcher. 1995. Cell 80:413. 5. Leitinger, B., et al., 2002, J. Cell. Sci. 115:963-72. PMID: 11870215 6. McDowall, A., et al., 2003, J Clin Invest. 111 (1):51-60. PMID: 12511588
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Product Notes
The CD49d/VLA-4 (Catalog #AAA670438) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CD49d/VLA-4-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD49d/VLA-4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blot (WB). Flow cytometry, Immunoprecipitation, Western blotting Flow Cytometry: <=1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD49d/VLA-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49d/VLA-4, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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