Mouse HLA-DP Antibody | anti-HLA-DPB1 antibody

HLA-DP (MHC II) Mouse Monoclonal Antibody

Cat. Num. AAA438873

• Gene Names: HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• Reactivity: Human. Others not known.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: HLA-DP; HLA-DP (MHC II) Mouse Monoclonal Antibody; HLA class II histocompatibility antigen DP alpha 1 chain; HLA DP1A; HLA SB alpha chain; HLADP; HLADPA1; HLASB; HLASB histocompatibility type; Human MHC class II HLA SB alpha; Major histocompatibility complex class II DP alpha 1; MHC class II DP3 alpha; MHC class II DPA1; PLT1; Primed lymphocyte test 1; anti-HLA-DPB1 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Isotype: IgG2b, kappa
• Clone Number: SPM421
• Specificity: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-HLA-DPB1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: ELISA (For coating use Ab at 2-4ug/ml, order antibody without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Optimal dilution for a specific application should be determined.
• Human Chromosome Location: 6p21.3
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Cellular Localization: Cell surface
• Positive Control: Raji cells. Tonsil or lymph node.
• Preparation and Storage: Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of Human Raji cells using HLA-DP (MHC II) Monoclonal Antibody (SPM421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

References

Babusikova O; Ujhazy P; Hrivnakova A; Chorvath B; Polakova K. Studies on the distribution of the antigens detected by some newly prepared monoclonal antibodies in normal hemopoietic and leukemic cells. Neoplasma, 1985, 32(6):657-62. Polakova K; Chorvath B; Sedlak J; Duraj J; Matoska J; Karpatova M. Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line. Neoplasma, 1985, 32(6):641-8. Neoplasma 34(4), 417- 425, (1987). Horejsi V, et. al. Tissue Antigens, 1988, 32(1):6-11.

NCBI and Uniprot Product Information

• NCBI GI #: 24797076
• NCBI GeneID: 3115
• NCBI Accession #: NP_002112.3
• NCBI GenBank Nucleotide #: NM_002121.5
• UniProt Accession #: P04440; A0PFJ7; A5I886; A8YPB3; B5U8B4; B7VF80; B7VF87; B8ZX68; B8ZYT0; B9W5S8; B9W6F7; B9W6F9
• Molecular Weight: 33-35 kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP beta 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP beta 1
• NCBI Official Symbol: HLA-DPB1
• NCBI Official Synonym Symbols: DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• NCBI Protein Information: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DPB1
• UniProt Synonym Gene Names: HLA-DP1B
• UniProt Entry Name: DPB1_HUMAN

NCBI Description

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DPB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; Golgi membrane; lysosomal membrane; membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane

Molecular Function: peptide antigen binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of interferon-gamma production; positive regulation of T cell activation; positive regulation of T cell proliferation; T cell costimulation; T cell receptor signaling pathway