Mouse anti-Human Granzyme B (NK/T-Cell Lymphoma Marker) Antibody | anti-GZMB antibody

Granzyme B (NK/T-Cell Lymphoma Marker) Mouse Monoclonal Antibody

Cat. Num. AAA4380760

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Granzyme B (NK/T-Cell Lymphoma Marker); Granzyme B (NK/T-Cell Lymphoma Marker) Mouse Monoclonal Antibody; Cathepsin G-like 1; CCPI; CGL1; CSPB; CTLA-1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; Granzyme B; Granzyme-2; GRB; Gzmb; Human lymphocyte protein (Hlp); Lymphocyte protease; SECT; T-cell serine protease 1-3E; anti-GZMB antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2b, k
• Clone Number: GZMB/3014
• Specificity: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin’s Disease.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-GZMB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blotting (1-2ug/ml); Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary
• Cellular Localization: Cytoplasmic
• Positive Control: A431 cells. Tonsil, Spleen or Hodgkin’s Lymphoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Spleen stained with Granzyme B Mouse Monoclonal Antibody (GZMB/3014).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Granzyme B Mouse Monoclonal Antibody (GZMB/3014).)

SDS-Page

(SDS-PAGE Analysis Purified Granzyme B Mouse Monoclonal Antibody (GZMB/3014). Confirmation of Integrity and Purity of Antibody.)

Testing Data

(Analysis of Protein Array containing >19,000 full-length human proteins using Granzyme B Mouse Monoclonal Antibody (GZMB/3014))

References

1. Shresta, S., et al. 1995. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells. Proc. Natl. Acad. Sci. USA 92: 5679-5683.

NCBI and Uniprot Product Information

• NCBI GeneID: 3002
• UniProt Accession #: P10144
• Molecular Weight: 29-32kDa
• Protein Family: Granzyme