Mouse Zinc finger protein ZIC 1 (ZIC1) ELISA Kit | ZIC1 elisa kit

Mouse Zinc finger protein ZIC 1 (ZIC1) ELISA Kit

Cat. Num. AAA7237747

• Gene Names: ZIC1; ZIC; ZNF201
• Reactivity: Mouse
• Synonyms: Zinc finger protein ZIC 1 (ZIC1); Mouse Zinc finger protein ZIC 1 (ZIC1) ELISA Kit; ZIC1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ZIC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ZIC1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for ZIC1 elisa kit

Principle of the Assay: ZIC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZIC1 antibody and an ZIC1-HRP conjugate. The assay sample and buffer are incubated together with ZIC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZIC1 concentration since ZIC1 from samples and ZIC1-HRP conjugate compete for the anti-ZIC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZIC1 from the sample, fewer sites are left to bind ZIC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZIC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ZIC1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 22547182
• NCBI GeneID: 7545
• NCBI Accession #: NP_003403.2
• NCBI GenBank Nucleotide #: NM_003412.3
• Molecular Weight: 48,309 Da
• NCBI Official Full Name: zinc finger protein ZIC 1
• NCBI Official Synonym Full Names: Zic family member 1
• NCBI Official Symbol: ZIC1
• NCBI Official Synonym Symbols: ZIC; ZNF201
• NCBI Protein Information: zinc finger protein ZIC 1; zinc finger protein 201; Zinc finger protein of the cerebellum 1; Zic family member 1 (odd-paired homolog, Drosophila)
• UniProt Protein Name: Zinc finger protein ZIC 1
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC1
• UniProt Synonym Gene Names: ZIC; ZNF201
• UniProt Entry Name: ZIC1_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZIC1: Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally- located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'- TGGGTGGTC-3'. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: inner ear morphogenesis; positive regulation of protein import into nucleus; transcription, DNA-dependent; spinal cord development; positive regulation of transcription, DNA-dependent; brain development; cell differentiation; adult walking behavior; pattern specification process; regulation of smoothened signaling pathway