Mouse VEGF-A ELISA (total) ELISA Kit | VEGF-A ELISA elisa kit

Mouse VEGF-A ELISA (total)

Cat. Num. AAA691463

• Gene Names: vegfaa; vegf; vegfa; wu:fj82c06
• Reactivity: Mouse
• Synonyms: VEGF-A ELISA (total); Mouse VEGF-A ELISA (total); VEGFA; VPF; VEGF; MVCD1; VEGF-A ELISA elisa kit

• Reactivity: Mouse
• Form/Format: Box containing complete ELISA set
• Sequence Length: 144

Related Product Information for VEGF-A ELISA elisa kit

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165). All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. In cultured endothelial cells, VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. In vivo, VEGF can induce angiogenesis as well as increase microvascular permeability. As a vascular permeability factor, VEGF acts directly on the endothelium and does not degranulate mast cells. Based on its in vitro and in vivo properties, VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 167736373
• NCBI GeneID: 30682
• NCBI Accession #: NP_001103819.2
• NCBI GenBank Nucleotide #: NM_001110349.2
• UniProt Accession #: O73682; O73822; B3DG34
• Molecular Weight: 21,756 Da
• NCBI Official Full Name: vascular endothelial growth factor A-A isoform 2
• NCBI Official Synonym Full Names: vascular endothelial growth factor Aa
• NCBI Official Symbol: vegfaa
• NCBI Official Synonym Symbols: vegf; vegfa; wu:fj82c06
• NCBI Protein Information: vascular endothelial growth factor A-A; vegf-a; VEGF-A-A; etID309976.16
• UniProt Protein Name: Vascular endothelial growth factor A-A
• UniProt Gene Name: vegfaa
• UniProt Synonym Gene Names: vegf; vegfa; VEGF-A-A
• UniProt Entry Name: VGFAA_DANRE

Uniprot Description

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Acts both upstream of kdr and tie1 to stimulate endothelial cell differentiation, and upstream of gata1 to stimulate hematopoietic cell differentiation. Ref.2 Ref.4 UniProtKB P15692

Subunit structure: Homodimer; disulfide-linked

By similarity. Isoform VEGF165 binds kdr and kdrl. UniProtKB P15692

Subcellular location: Secreted Ref.4.

Tissue specificity: Predominantly expressed in regions associated with active vascularization. From 15-16 hours post-fertilization (hpf), expressed in the anterior forebrain, the mesoderm underlying and lateral to the anterior hindbrain, the mesoderm underlying and lateral to the posterior hindbrain, and in the ventral medial portions of the somites. By 30-36 hpf, expression in the somites is decreased, while strong expression is observed in the region of the developing glomeruli and in the anterior portion of the pronephric ducts, the pharyngeal arches, and the brain. By 72 hpf, expression remains only in the pronephros region.

Developmental stage: Expressed both maternally and zygotically. Present throughout embryonic development, and in adults. Ref.1 Ref.2

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.