Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Uridine phosphorylase 1 (UPP1) Standard Curve.)
Mouse Uridine Phosphorylase 1 (UPP1) ELISA Kit | UPP1 elisa kit
Mouse Uridine Phosphorylase 1 (UPP1) ELISA Kit
Gene Names
Upp1; Up; Upp; UPase; UdRPase; AI325217
Reactivity
Mouse
Synonyms
Uridine Phosphorylase 1 (UPP1); Mouse Uridine Phosphorylase 1 (UPP1) ELISA Kit; UPP1; UDRPASE; UP; UPASE; UPP; OTTHUMP00000159566; UPP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UPP1. No significant cross-reactivity or interference between Mouse UPP1 and analogues was observed.
Sequence Length
311
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Uridine phosphorylase 1 (UPP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Uridine phosphorylase 1 (UPP1) Standard Curve.)
Related Product Information for UPP1 elisa kit
Description: This Mouse Uridine phosphorylase 1 (UPP1) ELISA Kit employs a two-site sandwich ELISA to quantitate UPP1 in samples. An antibody specific for UPP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UPP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UPP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UPP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Uridine Phosphorylase 2 belongs to the PNP/UDP phosphorylase family.Predominantly expressed in kidney.Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine.This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase.
Background: Uridine Phosphorylase 2 belongs to the PNP/UDP phosphorylase family.Predominantly expressed in kidney.Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine.This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
uridine phosphorylase 1
NCBI Official Synonym Full Names
uridine phosphorylase 1
NCBI Official Symbol
Upp1
NCBI Official Synonym Symbols
Up; Upp; UPase; UdRPase; AI325217
NCBI Protein Information
uridine phosphorylase 1
UniProt Protein Name
Uridine phosphorylase 1
Protein Family
UniProt Gene Name
Upp1
UniProt Synonym Gene Names
Up; Upp; UPase 1; UrdPase 1
Uniprot Description
UPP1: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Belongs to the PNP/UDP phosphorylase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.4.2.3; Nucleotide Metabolism - pyrimidine; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes
Chromosomal Location of Human Ortholog: 11|11 A1
Cellular Component: cytoplasm
Molecular Function: catalytic activity; transferase activity; transferase activity, transferring glycosyl groups; transferase activity, transferring pentosyl groups; uridine phosphorylase activity
Biological Process: nucleoside metabolic process; nucleotide catabolic process; pyrimidine nucleotide metabolic process; uridine catabolic process; uridine metabolic process
Research Articles on UPP1
Similar Products
Product Notes
The Mouse UPP1 upp1 (Catalog #AAA9716646) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716646 ELISA Kit recognizes Mouse UPP1. It is sometimes possible for the material contained within the vial of "Uridine Phosphorylase 1 (UPP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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