Mouse Ubiquitin ELISA Kit

Mouse Ubiquitin ELISA Kit

Cat. Num. AAA3806338

• Reactivity: Mouse
• Synonyms: Ubiquitin; Mouse Ubiquitin ELISA Kit; Ubiquitin elisa kit

• Reactivity: Mouse
• Sequence Length: 76

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 12.5ng/ml-200ng/ml
• Sensitivity: 1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ubiquitin elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofUBIQUITINin the sample, This UBIQUITINELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusUBIQUITINconcentration. The concentration ofUBIQUITINinthe samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 136667
• UniProt Accession #: P19848
• NCBI Official Full Name: Ubiquitin
• UniProt Protein Name: Ubiquitin
• Protein Family: Ubiquitin

Uniprot Description

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling ().