Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tryptophan 5-hydroxylase 2 (TPH2) Standard Curve.)
Mouse Tryptophan 5-Hydroxylase 2 (TPH2) ELISA Kit | TPH2 elisa kit
Mouse Tryptophan 5-Hydroxylase 2 (TPH2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tryptophan 5-hydroxylase 2 (TPH2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tryptophan 5-hydroxylase 2 (TPH2) Standard Curve.)
Background: TPH2 encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by the mouse ortholog.
NCBI and Uniprot Product Information
Uniprot Description
TPH2: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described. Isoform 2 appears to be expressed exclusively in the brain, and is expressed at significantly higher levels in the raphe nuclei than isoform 1.
Protein type: Amino Acid Metabolism - tryptophan; EC 1.14.16.4; Oxidoreductase
Chromosomal Location of Human Ortholog: 10|10 D2
Cellular Component: neuron projection
Molecular Function: iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; tryptophan 5-monooxygenase activity
Biological Process: aromatic amino acid family metabolic process; serotonin biosynthetic process; serotonin biosynthetic process from tryptophan
Research Articles on TPH2
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Product Notes
The Mouse TPH2 tph2 (Catalog #AAA9716679) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716679 ELISA Kit recognizes Mouse TPH2. It is sometimes possible for the material contained within the vial of "Tryptophan 5-Hydroxylase 2 (TPH2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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