Mouse Troponin T Type 2, Cardiac (TNNT2) ELISA Kit | TNNT2 elisa kit

Mouse Troponin T Type 2, Cardiac (TNNT2) Mini Samples ELISA Kit

Cat. Num. AAA2708409

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Troponin T Type 2; Cardiac (TNNT2); Mouse Troponin T Type 2; Cardiac (TNNT2) Mini Samples ELISA Kit; Cardiac; CMH2; cTnT; TnTc; Cardiomyopathy; Hypertrophic 2; Cardiac muscle troponin T; TNNT2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Troponin T Type 2, Cardiac (TNNT2).; No significant cross-reactivity or interference between Mini Samples Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.
• Sequence Length: 285

• Applicable Applications for TNNT2 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Troponin T Type 2, Cardiac (TNNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Troponin T Type 2, Cardiac (TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Troponin T Type 2, Cardiac (TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Troponin T Type 2, Cardiac (TNNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 12803637
• NCBI GeneID: 7139
• NCBI Official Full Name: Troponin T type 2 (cardiac)
• NCBI Official Synonym Full Names: troponin T2, cardiac type
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle
• Protein Family: Troponin

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]