Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 66 (TMEM66) Standard Curve.)
Mouse Transmembrane Protein 66 (TMEM66) ELISA Kit | TMEM66 elisa kit
Mouse Transmembrane Protein 66 (TMEM66) ELISA Kit
Gene Names
Saraf; Tmem66; 1810045K07Rik
Reactivity
Mouse
Synonyms
Transmembrane Protein 66 (TMEM66); Mouse Transmembrane Protein 66 (TMEM66) ELISA Kit; TMEM66; FLJ22274; FOAP-7; HSPC035; MGC8721; XTP3; TMEM66 elisa kit
Reactivity
Mouse
Specificity
Mouse Transmembrane protein 66 (TMEM66) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM66. No significant cross-reactivity or interference between Mouse TMEM66 and analogues was observed.
Sequence Length
363
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 66 (TMEM66) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transmembrane protein 66 (TMEM66) Standard Curve.)
Related Product Information for TMEM66 elisa kit
Description: This Mouse Transmembrane protein 66 (TMEM66) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM66 in samples. An antibody specific for TMEM66 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM66 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM66 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM66 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully.
Background: Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
store-operated calcium entry-associated regulatory factor
NCBI Official Synonym Full Names
store-operated calcium entry-associated regulatory factor
NCBI Official Symbol
Saraf
NCBI Official Synonym Symbols
Tmem66; 1810045K07Rik
NCBI Protein Information
store-operated calcium entry-associated regulatory factor
UniProt Protein Name
Store-operated calcium entry-associated regulatory factor
UniProt Gene Name
Saraf
UniProt Synonym Gene Names
Tmem66; SARAF; SOCE-associated regulatory factor
UniProt Entry Name
SARAF_MOUSE
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Product Notes
The Mouse TMEM66 saraf (Catalog #AAA9716774) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716774 ELISA Kit recognizes Mouse TMEM66. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 66 (TMEM66), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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