Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF2 and NCK-interacting protein kinase (TNIK) Standard Curve.)
Mouse TRAF2 And NCK-Interacting Protein Kinase (TNIK) ELISA Kit | TNIK elisa kit
Mouse TRAF2 And NCK-Interacting Protein Kinase (TNIK) ELISA Kit
Gene Names
Tnik; AI451411; 1500031A17Rik; 4831440I19Rik; C530008O15Rik; C630040K21Rik
Reactivity
Mouse
Synonyms
TRAF2 And NCK-Interacting Protein Kinase (TNIK); Mouse TRAF2 And NCK-Interacting Protein Kinase (TNIK) ELISA Kit; TNIK elisa kit
Reactivity
Mouse
Specificity
Mouse TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNIK. No significant cross-reactivity or interference between Mouse TNIK and analogues was observed.
Sequence Length
1323
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF2 and NCK-interacting protein kinase (TNIK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF2 and NCK-interacting protein kinase (TNIK) Standard Curve.)
Related Product Information for TNIK elisa kit
Description: This Mouse TRAF2 and NCK-interacting protein kinase (TNIK) ELISA Kit employs a two-site sandwich ELISA to quantitate TNIK in samples. An antibody specific for TNIK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNIK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNIK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNIK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNIK was autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain. Immunoprecipitation analysis showed that epitope-tagged TNIK coprecipitated endogenous TRAF2 from human embryonic kidney cells. Mutation analysis revealed that the intermediate domain was sufficient for the interaction, although the GCK domain may contribute. The intermediate domain was also sufficient for interaction with NCK. Cotransfection of TNIK with JNK2 (MAPK9) enhanced JNK2 kinase activity in a dose-dependent manner, and this effect was mediated by the GCK region of TNIK, but not the kinase domain. TNIK overexpression had no effect on ERK1 (MAPK3), p38 (MAPK14), or NF-kappa-B.
Background: TNIK was autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain. Immunoprecipitation analysis showed that epitope-tagged TNIK coprecipitated endogenous TRAF2 from human embryonic kidney cells. Mutation analysis revealed that the intermediate domain was sufficient for the interaction, although the GCK domain may contribute. The intermediate domain was also sufficient for interaction with NCK. Cotransfection of TNIK with JNK2 (MAPK9) enhanced JNK2 kinase activity in a dose-dependent manner, and this effect was mediated by the GCK region of TNIK, but not the kinase domain. TNIK overexpression had no effect on ERK1 (MAPK3), p38 (MAPK14), or NF-kappa-B.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
traf2 and NCK-interacting protein kinase isoform 3
NCBI Official Synonym Full Names
TRAF2 and NCK interacting kinase
NCBI Official Symbol
Tnik
NCBI Official Synonym Symbols
AI451411; 1500031A17Rik; 4831440I19Rik; C530008O15Rik; C630040K21Rik
NCBI Protein Information
traf2 and NCK-interacting protein kinase
UniProt Protein Name
Traf2 and NCK-interacting protein kinase
Protein Family
UniProt Gene Name
Tnik
UniProt Synonym Gene Names
Kiaa0551
UniProt Entry Name
TNIK_MOUSE
Research Articles on TNIK
Similar Products
Product Notes
The Mouse TNIK tnik (Catalog #AAA9716722) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716722 ELISA Kit recognizes Mouse TNIK. It is sometimes possible for the material contained within the vial of "TRAF2 And NCK-Interacting Protein Kinase (TNIK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
