Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF7/CD27 PicoKine ELISA Kit standard curve )
Mouse TNFRSF7/CD27 ELISA Kit | CD27 elisa kit
Mouse TNFRSF7/CD27 PicoKine ELISA Kit
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Synonyms
TNFRSF7/CD27; Mouse TNFRSF7/CD27 PicoKine ELISA Kit; CD27 antigen; CD27 molecule; CD 27; Cd27; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7; CD27 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse TNFRSF7
Sequence Length
250
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF7/CD27 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse TNFRSF7/CD27 PicoKine ELISA Kit standard curve )
Related Product Information for CD27 elisa kit
Principle of the assay: mouse TNFRSF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF7 amount of sample captured in plate.
Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,164 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 antigen
NCBI Official Symbol
Cd27
NCBI Official Synonym Symbols
S152; Tp55; Tnfrsf7
NCBI Protein Information
CD27 antigen; CD antigen 27; CD27L receptor; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
UniProt Protein Name
CD27 antigen
Protein Family
UniProt Gene Name
Cd27
UniProt Synonym Gene Names
Tnfrsf7
UniProt Entry Name
CD27_MOUSE
Uniprot Description
CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis
Cellular Component: cell surface; membrane; cell; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; caspase inhibitor activity
Biological Process: B cell proliferation; positive regulation of T cell differentiation; apoptosis; immunoglobulin secretion; positive regulation of JNK cascade; negative regulation of caspase activity; negative regulation of apoptosis; positive regulation of interferon-gamma biosynthetic process
Research Articles on CD27
Similar Products
Product Notes
The Mouse CD27 cd27 (Catalog #AAA177365) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177365 ELISA Kit recognizes Mouse CD27. It is sometimes possible for the material contained within the vial of "TNFRSF7/CD27, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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