Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tensin-3 (TNS3) Standard Curve.)
Mouse Tensin-3 (TNS3) ELISA Kit | TNS3 elisa kit
Mouse Tensin-3 (TNS3) ELISA Kit
Gene Names
Tns3; TEM6; Tens1; BC023928; F830010I22Rik
Reactivity
Mouse
Synonyms
Tensin-3 (TNS3); Mouse Tensin-3 (TNS3) ELISA Kit; TNS3; DKFZp686K12123; DKFZp686M1045; FLJ13732; FLJ35545; H_NH049I23.2; MGC88434; TEM6; TENS1; H_NH0549I23.2; OTTHUMP00000082709; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing 1; thyro; TNS3 elisa kit
Reactivity
Mouse
Specificity
Mouse Tensin-3 (TNS3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNS3. No significant cross-reactivity or interference between Mouse TNS3 and analogues was observed.
Sequence Length
1440
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tensin-3 (TNS3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tensin-3 (TNS3) Standard Curve.)
Related Product Information for TNS3 elisa kit
Description: This Mouse Tensin-3 (TNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS3 in samples. An antibody specific for TNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells. Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR. The deduced 1,445-amino acid protein has a calculated molecular mass of 155.3 kD. Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains. It also has several potential tyrosine phosphorylation sites. Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung. Western blot analysis revealed a 180-kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.
Background: Tensin-3 formed a complex with focal adhesion kinase and p130Cas (BCAR1) in human breast carcinoma cells. Addition of EGF to these cells induced dephosphorylation of FAK and p130Cas, leading to dissociation of the tensin-3/FAK/p130Cas complex and enhanced interaction between tensin-3 and EGFR. The deduced 1,445-amino acid protein has a calculated molecular mass of 155.3 kD. Like other tensins, tensin-3 has an N-terminal actin-binding domain and C-terminal SH2 and PTB domains. It also has several potential tyrosine phosphorylation sites. Northern blot analysis detected high expression of an 8-kb transcript in kidney and placenta, with weaker expression in heart, skeletal muscle, spleen, liver, and lung. Western blot analysis revealed a 180-kD tensin-3 protein in human endothelial and epithelial cells and fibroblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tensin-3
NCBI Official Synonym Full Names
tensin 3
NCBI Official Symbol
Tns3
NCBI Official Synonym Symbols
TEM6; Tens1; BC023928; F830010I22Rik
NCBI Protein Information
tensin-3
UniProt Protein Name
Tensin-3
Protein Family
UniProt Gene Name
Tns3
UniProt Synonym Gene Names
Tens1
UniProt Entry Name
TENS3_MOUSE
Research Articles on TNS3
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Product Notes
The Mouse TNS3 tns3 (Catalog #AAA9716696) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716696 ELISA Kit recognizes Mouse TNS3. It is sometimes possible for the material contained within the vial of "Tensin-3 (TNS3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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