Mouse T cell immunoreceptor with Ig and ITIM domains ELISA Kit | Tigit elisa kit

Mouse T cell immunoreceptor with Ig and ITIM domains, TIGIT ELISA Kit

Cat. Num. AAA2602968

• Gene Names: Tigit; Vstm3; ENSMUSG00000071552
• Reactivity: Mouse
• Synonyms: T cell immunoreceptor with Ig and ITIM domains; Mouse T cell immunoreceptor with Ig and ITIM domains; TIGIT ELISA Kit; Tigit elisa kit

• Reactivity: Mouse

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Tigit elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse TIGIT monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 224493410
• NCBI GeneID: 100043314
• UniProt Accession #: P86176
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: Tigit
• NCBI Official Synonym Symbols: Vstm3; ENSMUSG00000071552
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• Protein Family: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: Tigit
• UniProt Synonym Gene Names: Vstm3
• UniProt Entry Name: TIGIT_MOUSE

Uniprot Description

TIGIT: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: cell surface

Molecular Function: receptor binding

Biological Process: negative regulation of interleukin-12 production; negative regulation of T cell activation; positive regulation of interleukin-10 production