Mouse Sulfatase 2 (SULF2) ELISA Kit | SULF2 elisa kit

Mouse Sulfatase 2 (SULF2) ELISA Kit

Cat. Num. AAA2000204

• Gene Names: SULF2; HSULF-2
• Reactivity: Mouse
• Synonyms: Sulfatase 2 (SULF2); Mouse Sulfatase 2 (SULF2) ELISA Kit; HSULF-2; Extracellular sulfatase Sulf-2; SULF2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sulfatase 2 (SULF2).; No significant cross-reactivity or interference between Sulfatase 2 (SULF2) and analogues was observed.
• Sequence Length: 870

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SULF2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfatase 2 (SULF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfatase 2 (SULF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfatase 2 (SULF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sulfatase 2 (SULF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SULF2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 83405533
• NCBI GeneID: 55959
• UniProt Accession #: Q8IWU5; Q5JYE1; Q6UX86; Q96SG2; Q9H1H0; Q9UJR3; Q9ULH3; E1P5U6
• Molecular Weight: 100,156 Da
• NCBI Official Full Name: Sulfatase 2
• NCBI Official Synonym Full Names: sulfatase 2
• NCBI Official Symbol: SULF2
• NCBI Official Synonym Symbols: HSULF-2
• NCBI Protein Information: extracellular sulfatase Sulf-2
• UniProt Protein Name: Extracellular sulfatase Sulf-2
• Protein Family: Extracellular sulfatase
• UniProt Gene Name: SULF2
• UniProt Synonym Gene Names: KIAA1247; hSulf-2
• UniProt Entry Name: SULF2_HUMAN

NCBI Description

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

Uniprot Description

SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.6.-; Hydrolase; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane

Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding

Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; embryonic skeletal development; cartilage development; glomerular basement membrane development; glomerular filtration; esophagus smooth muscle contraction; heparan sulfate proteoglycan metabolic process; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway