Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) Standard Curve.)
Mouse Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial (SDHA) ELISA Kit | SDHA elisa kit
Mouse Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial (SDHA) ELISA Kit
Gene Names
Sdha; FP; SDH2; SDHF; C81073; 4921513A11; 1500032O14Rik; 2310034D06Rik
Reactivity
Mouse
Synonyms
Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit; Mitochondrial (SDHA); Mouse Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit; Mitochondrial (SDHA) ELISA Kit; SDHA; FP; SDH1; SDH2; SDHF; succinate dehydrogenase complex flavoprotein subunit; succinate dehydrogenase complex; subunit A; flavoprotein; SDHA elisa kit
Reactivity
Mouse
Specificity
Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHA. No significant cross-reactivity or interference between Mouse SDHA and analogues was observed.
Sequence Length
664
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) Standard Curve.)
Related Product Information for SDHA elisa kit
Description: This Mouse Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHA in samples. An antibody specific for SDHA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDHA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SDHA encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Hirawake et al. (1994) isolated a cDNA corresponding to the flavoprotein subunit of SDH from a human liver cDNA library. The deduced 621-amino acid protein has a molecular mass of approximately 68 kD and shows high homology to the bovine heart flavoprotein subunit.
Background: SDHA encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Hirawake et al. (1994) isolated a cDNA corresponding to the flavoprotein subunit of SDH from a human liver cDNA library. The deduced 621-amino acid protein has a molecular mass of approximately 68 kD and shows high homology to the bovine heart flavoprotein subunit.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
NCBI Official Symbol
Sdha
NCBI Official Synonym Symbols
FP; SDH2; SDHF; C81073; 4921513A11; 1500032O14Rik; 2310034D06Rik
NCBI Protein Information
succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Protein Family
UniProt Gene Name
Sdha
UniProt Synonym Gene Names
Fp
Uniprot Description
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Research Articles on SDHA
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Product Notes
The Mouse SDHA sdha (Catalog #AAA9717078) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717078 ELISA Kit recognizes Mouse SDHA. It is sometimes possible for the material contained within the vial of "Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial (SDHA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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