Mouse SPON2 (Spondin-2) ELISA Kit | SPON2 elisa kit

Mouse SPON2 (Spondin-2) ELISA Kit

Cat. Num. AAA7612388

• Gene Names: Spon2; Mindin; AI504350; Mspondin; M-spondin; 2310045I24Rik
• Reactivity: Mouse
• Synonyms: SPON2 (Spondin-2); Mouse SPON2 (Spondin-2) ELISA Kit; Spondin-2; Mindin; Spon2; SPON2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPON2. No significant cross-reactivity or interference between SPON2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPON2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 254939623
• NCBI GeneID: 100689
• NCBI Accession #: NP_598664.3
• NCBI GenBank Nucleotide #: NM_133903.3
• UniProt Accession #: Q8BMS2; Q6SJD8
• Molecular Weight: 35,965 Da
• NCBI Official Full Name: spondin-2
• NCBI Official Synonym Full Names: spondin 2, extracellular matrix protein
• NCBI Official Symbol: Spon2
• NCBI Official Synonym Symbols: Mindin; AI504350; Mspondin; M-spondin; 2310045I24Rik
• NCBI Protein Information: spondin-2
• UniProt Protein Name: Spondin-2
• Protein Family: Spondin
• UniProt Gene Name: Spon2
• UniProt Entry Name: SPON2_MOUSE

Uniprot Description

SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: antigen binding; lipopolysaccharide binding; metal ion binding; protein binding; receptor binding

Biological Process: cell adhesion; defense response to bacterium; defense response to fungus; defense response to virus; immune system process; induction of bacterial agglutination; innate immune response; mast cell mediated immunity; opsonization; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; response to lipopolysaccharide