Mouse SPARC ELISA Kit | Sparc elisa kit

Mouse SPARC ELISA Kit

Cat. Num. AAA763728

• Gene Names: SPARC; ON; OI17; BM-40
• Reactivity: Mouse
• Synonyms: SPARC; Mouse SPARC ELISA Kit; Sparc/SPARC/BM-40/Osteonectin/Basement-membrane protein 40/BM-40/ONcysteine-rich protein/osteonectin/Osteonectin/Secreted protein acidic and rich in cysteine/secreted protein; acidic; cysteine-rich (osteonectin); Sparc elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sparc. No significant cross-reactivity or interference between Sparc and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between Sparc and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 303

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12-200ng/ml
• Sensitivity: <1.875ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sparc were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sparc were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for Sparc elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Sparc antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Sparc antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Sparc amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Sparc can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507171
• NCBI GeneID: 6678
• NCBI Accession #: NP_003109.1
• NCBI GenBank Nucleotide #: NM_003118.3
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: SPARC isoform 1
• NCBI Official Synonym Full Names: secreted protein acidic and cysteine rich
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON; OI17; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: basement membrane; cell surface; cytoplasm; extracellular region; extracellular space; mitochondrion; nuclear matrix; plasma membrane; platelet alpha granule membrane

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; protein binding

Biological Process: blood coagulation; extracellular matrix organization and biogenesis; heart development; inner ear development; lung development; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; ossification; platelet activation; platelet degranulation; receptor-mediated endocytosis; regulation of cell morphogenesis; response to cadmium ion; response to calcium ion; response to cAMP; response to cytokine stimulus; response to ethanol; response to glucocorticoid stimulus; response to gravity; response to L-ascorbic acid; response to lead ion; response to lipopolysaccharide; response to peptide hormone stimulus; signal transduction

Disease: Osteogenesis Imperfecta, Type Xvii