Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.)
Mouse Sorbin And SH3 Domain-Containing Protein 2 (SORBS2) ELISA Kit | SORBS2 elisa kit
Mouse Sorbin And SH3 Domain-Containing Protein 2 (SORBS2) ELISA Kit
Gene Names
Sorbs2; Argbp2; nArgBP2; mKIAA0777; A530071H08; 2010203O03Rik; 9430041O17Rik
Reactivity
Mouse
Synonyms
Sorbin And SH3 Domain-Containing Protein 2 (SORBS2); Mouse Sorbin And SH3 Domain-Containing Protein 2 (SORBS2) ELISA Kit; SORBS2; ARGBP2; FLJ93447; KIAA0777; PRO0618; Arg binding protein 2; Arg/Abl interacting protein; Arg/Abl-interacting protein 2; OTTHUMP00000211726; SORBS2 elisa kit
Reactivity
Mouse
Specificity
Mouse Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SORBS2. No significant cross-reactivity or interference between Mouse SORBS2 and analogues was observed.
Sequence Length
707
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorbin and SH3 domain-containing protein 2 (SORBS2) Standard Curve.)
Related Product Information for SORBS2 elisa kit
Description: This Mouse Sorbin and SH3 domain-containing protein 2 (SORBS2) ELISA Kit employs a two-site sandwich ELISA to quantitate SORBS2 in samples. An antibody specific for SORBS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SORBS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORBS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORBS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. SORBS2 encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms.
Background: Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. SORBS2 encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sorbin and SH3 domain-containing protein 2 isoform 3
NCBI Official Synonym Full Names
sorbin and SH3 domain containing 2
NCBI Official Symbol
Sorbs2
NCBI Official Synonym Symbols
Argbp2; nArgBP2; mKIAA0777; A530071H08; 2010203O03Rik; 9430041O17Rik
NCBI Protein Information
sorbin and SH3 domain-containing protein 2
UniProt Protein Name
Sorbin and SH3 domain-containing protein 2
Protein Family
UniProt Gene Name
Sorbs2
UniProt Synonym Gene Names
Argbp2; Kiaa0777; ArgBP2
Uniprot Description
ArgBP2: Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8|8 B1.1
Cellular Component: cell junction; cell projection; cell soma; cytoplasm; cytoskeleton; dendrite; membrane; plasma membrane; postsynaptic density
Molecular Function: nucleic acid binding; protein dimerization activity; protein domain specific binding; RNA binding
Biological Process: actin filament organization; cell adhesion; cell migration; Notch signaling pathway
Research Articles on SORBS2
Similar Products
Product Notes
The Mouse SORBS2 sorbs2 (Catalog #AAA9717017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717017 ELISA Kit recognizes Mouse SORBS2. It is sometimes possible for the material contained within the vial of "Sorbin And SH3 Domain-Containing Protein 2 (SORBS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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