Typical Testing Data/Standard Curve (for reference only)
Mouse SerpinE2 ELISA Kit | SERPINE2 elisa kit
Mouse SerpinE2 PicoKine ELISA Kit
Gene Names
SERPINE2; GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
Reactivity
Mouse
Synonyms
SerpinE2; Mouse SerpinE2 PicoKine ELISA Kit; Mouse SerpinE2; SERPINE2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Serpine2
Sequence Length
397
Samples
Cell culture supernates, serum and plasma(heparin, edta, citrate),urine
Detection Range
0.78ng/ml-50ng/ml
Sensitivity
<0.1ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINE2 elisa kit
Principle of the Assay: The Picokine™ Mouse Serpine2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Serpine2 with a 96-well strip plate that is pre-coated with antibody specific for Serpine2. The detection antibody is a biotinylated antibody specific for Serpine2. The capture antibody is A polyclonal antibody from goat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse Serpine2 with immunogen: Expression system for standard: NSO, Immunogen sequence: S20-P397. The kit is analytically validated with ready to use reagents. To measure Mouse Serpine2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Serpine2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Serpine2 in the sample.
Background/Introduction: Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atCTerminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
Background/Introduction: Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atCTerminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
SERPINE2 protein
NCBI Official Synonym Full Names
serpin family E member 2
NCBI Official Symbol
SERPINE2
NCBI Official Synonym Symbols
GDN; PI7; PN1; PNI; PI-7; PN-1; GDNPF
NCBI Protein Information
glia-derived nexin
Protein Family
NCBI Description
This gene encodes a member of the serpin family of proteins, a group of proteins that inhibit serine proteases. Thrombin, urokinase, plasmin and trypsin are among the proteases that this family member can inhibit. This gene is a susceptibility gene for chronic obstructive pulmonary disease and for emphysema. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Research Articles on SERPINE2
Similar Products
Product Notes
The Mouse SERPINE2 (Catalog #AAA1751667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751667 ELISA Kit recognizes Mouse SERPINE2. It is sometimes possible for the material contained within the vial of "SerpinE2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
