Mouse SAP ELISA Kit | APCS elisa kit
Mouse SAP ELISA Kit
Gene Names
APCS; MGC127839
Reactivity
Mouse
Synonyms
SAP; Mouse SAP ELISA Kit; Mouse SAP; APCS elisa kit
Reactivity
Mouse
Samples
Immunoperoxidase Assay for Determination of SAP in Mouse Samples
Assay Type
Sandwich
Intended Uses
The SAP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAP in biological samples of mice.
Preparation and Storage
Store at 4 degree C.
Related Product Information for APCS elisa kit
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAP present in samples reacts with the anti-SAP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Background: Mouse serum amyloid P (SAP) is a major acute phase protein that circulates as an ~230 kDa glycoprotein. It is composed of 10 identical subunits arranged as two cyclic pentameric discs and contains ~10% carbohydrate. Basal levels of SAP may vary significantly in different inbred mouse strains but SAP is a major acute phase protein in all strains. During acute chemical, physical or inflammatory stimulus, its concentration can increase 50-100 fold within 24-48 hours. Changes can readily be detected in 4-6 hours. Measurement of the concentration of SAP is indicative of the extent and severity of an inflammatory stimulus and can be used to assess various modalities of treatment.
Background: Mouse serum amyloid P (SAP) is a major acute phase protein that circulates as an ~230 kDa glycoprotein. It is composed of 10 identical subunits arranged as two cyclic pentameric discs and contains ~10% carbohydrate. Basal levels of SAP may vary significantly in different inbred mouse strains but SAP is a major acute phase protein in all strains. During acute chemical, physical or inflammatory stimulus, its concentration can increase 50-100 fold within 24-48 hours. Changes can readily be detected in 4-6 hours. Measurement of the concentration of SAP is indicative of the extent and severity of an inflammatory stimulus and can be used to assess various modalities of treatment.
Product Categories/Family for APCS elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,183 Da
NCBI Official Full Name
serum amyloid P-component
NCBI Official Symbol
APCS
NCBI Official Synonym Symbols
MGC127839
NCBI Protein Information
serum amyloid P-component; SAP
UniProt Protein Name
Serum amyloid P-component
Protein Family
UniProt Gene Name
APCS
UniProt Entry Name
SAMP_BOVIN
Uniprot Description
Cofactor: Binds 2 calcium ions per subunit
Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.
Subcellular location: Secreted
Involvement in disease: Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits
Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.
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Product Notes
The Mouse APCS apcs (Catalog #AAA564086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA564086 ELISA Kit recognizes Mouse APCS. It is sometimes possible for the material contained within the vial of "SAP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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