Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit | RGS6 elisa kit

Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit

Cat. Num. AAA457151

• Reactivity: Mouse
• Synonyms: Regulator Of G Protein Signaling 6 (RGS6); Mouse Regulator Of G Protein Signaling 6 (RGS6) ELISA Kit; GAP; S914; RGS6 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of RGS6. No significant cross-reactivity or interference between RGS6 and analogues was observed.
• Sequence Length: 472

• Samples: Tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.066ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RGS6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RGS6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RGS6 in mouse tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RGS6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RGS6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RGS6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RGS6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 530678008
• NCBI GeneID: 50779
• NCBI Accession #: NP_001268990.1
• NCBI GenBank Nucleotide #: NM_001282061.2
• UniProt Accession #: Q9Z2H2; Q08AT5; Q8BGI8
• Molecular Weight: 54,531 Da
• NCBI Official Full Name: regulator of G-protein signaling 6 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 6
• NCBI Official Symbol: Rgs6
• NCBI Protein Information: regulator of G-protein signaling 6
• UniProt Protein Name: Regulator of G-protein signaling 6
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: Rgs6
• UniProt Synonym Gene Names: RGS6

NCBI Description

This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP (Dishevelled, Egl-10, and Pleckstrin) and GGL (G-protein gamma like)domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Mice lacking this gene exhibit decreased heart rate. Alternative splicing results in multiple transcript variants, however, the full-length nature of some of these variants is not known. [provided by RefSeq, Sep 2015]

Uniprot Description

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity.